Difference between revisions of "Van Bergen 2014 Mitochondrion"
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{{Publication | {{Publication | ||
|title=Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion | |title=Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/24657935 PMID: 24657935] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/24657935 PMID: 24657935] | ||
|authors=Van Bergen NJ, Blake RE, Crowston JG, Trounce IA | |authors=Van Bergen NJ, Blake RE, Crowston JG, Trounce IA | ||
| Line 11: | Line 11: | ||
|area=Respiration, Instruments;methods, mtDNA;mt-genetics, mt-Medicine | |area=Respiration, Instruments;methods, mtDNA;mt-genetics, mt-Medicine | ||
|organism=Human, Mouse | |organism=Human, Mouse | ||
|tissues=Nervous system | |tissues=Nervous system, Blood cells | ||
|preparations=Permeabilized cells, Isolated mitochondria, Intact cells | |||
|preparations= | |enzymes=Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Marker enzyme | ||
|enzymes=Complex I, Complex II; | |||
|topics=ATP production, Uncoupler | |topics=ATP production, Uncoupler | ||
|couplingstates=LEAK, ROUTINE, OXPHOS, | |couplingstates=LEAK, ROUTINE, OXPHOS, ET | ||
| | |pathways=N, S, NS | ||
|instruments=Oxygraph-2k, TIP2k, Protocol | |instruments=Oxygraph-2k, TIP2k, O2k-Protocol | ||
|additional=MitoEAGLE blood cells reviews, | |||
}} | }} | ||
== O2k-Publications == | |||
*[[O2k-Publications: Blood cells]] | |||
*[[O2k-Publications: Lymphocyte]] | |||
>>[[Bioblast_alert_2014]](02) | >>[[Bioblast_alert_2014]](02) | ||
Latest revision as of 09:18, 3 March 2020
| Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33. |
Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Mitochondrion
Abstract: Mitochondrial oxidative phosphorylation (OXPHOS) dysfunction is implicated in a growing spectrum of diseases, from neurodegeneration to cancer. Where tissues or transformed cells are available, respirometry and enzymology allow a sophisticated analysis of OXPHOS with modest-cost equipment. The isolation of organelle fractions is also invaluable for determining association of proteins of interest. Here we revisit and consolidate methods to measure whole cell mitochondrial ATP synthesis, respiration, isolation of mitochondria from cultured cells and tissues, and OXPHOS enzymology. We also explain common pitfalls, guide optimisation of the methods for new users, and provide full laboratory protocols in Supplementary materials.
• O2k-Network Lab: AU Melbourne Trounce IA
Labels: MiParea: Respiration, Instruments;methods, mtDNA;mt-genetics, mt-Medicine
Organism: Human, Mouse
Tissue;cell: Nervous system, Blood cells
Preparation: Permeabilized cells, Isolated mitochondria, Intact cells
Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Marker enzyme
Regulation: ATP production, Uncoupler
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, S, NS
HRR: Oxygraph-2k, TIP2k, O2k-Protocol
MitoEAGLE blood cells reviews
O2k-Publications
>>Bioblast_alert_2014(02)
