Template:SUIT-005
| Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
|---|---|---|---|---|
| 1OctM | OctML | F | CI | OctML or OctM_L: Octanoylcarnitine & low malate, N-LEAK respiration, NL
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. Low concentration of malate, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. Template:SUIT L n |
| 2D | OCtMP | F | CI | 1OCtM;2D
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. Low concentration of malate, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
| 2c | OCtMP | F | CI | 1OCtM;2D;2c
NADH-linked substrates (type N-pathway to Q). Low concentration of malate, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]). |
| 3P | OCtPMP | FN | CI&II | 1PM;2D;2c;3P
NADH-linked substrates (type N-pathway to Q). Template:SUIT P OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
| 4S | OCtPMSP | FNS | CI&II | 1PM;2D;2c;3P;4S
NADH-linked substrates (type N-pathway to Q). Template:SUIT P Succinate, S ( type S-pathway to Q). Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
| 5U | OCtPMSE | FNS | CI&II | 1PM;2D;2c;3P;4S;5U
NADH-linked substrates (type N-pathway to Q). Template:SUIT P Succinate, S ( type S-pathway to Q). Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. Noncoupled electron transfer state, ET state, with ET capacity E. |
| 6Rot | SE | S | CII | 1PM;2D;3P;4S;5U;6Rot
Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway. Noncoupled electron transfer state, ET state, with ET capacity E. |
| 7Ama | ROX | 1PM;2D;3P;4S;5U;6Rot;7Ama |
