Difference between revisions of "Template:SUIT-003 O2 ce D012"
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| Β | | Β | ||
| Β | | Β | ||
| ce1;ce2P | | ce1;ce2P Pyruvate, ROUTINE | ||
{{Template:SUIT ROUTINE}} | {{Template:SUIT ROUTINE}} | ||
Revision as of 10:28, 21 January 2019
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state. | ||
ce2P | ROUTINE | ce1;ce2P Pyruvate, ROUTINE
ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state. | ||
ce3Omy | L | ce1;ce2P;ce3Omy
| ||
ce4U | E | ce1;ce2P;ce3Omy;ce4U
Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E. | ||
ce* | ROX | ce1;ce2P;ce3Omy;ce4U;ce*
Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. |