Template:Next oroboros ecosystem agenda: Difference between revisions
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|'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed? | |'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed? | ||
Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br> | Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br> | ||
- »[ | - »[[Instrumental:_Browse_DL-Protocols_and_templates|How to find the background H<sub>2</sub>O<sub>2</sub> calibration protocol in DatLab]] « select “AmR calibration” for the background H<sub>2</sub>O<sub>2</sub> calibration protocol <br> | ||
- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]« | - communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]« | ||
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* '''Oct-20 Tu''' - [ | * '''Oct-20 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]] | ||
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* '''Nov-03 Tu''' - [ | * '''Nov-03 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]] | ||
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* '''Nov-17 Tu''' - [ | * '''Nov-17 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]] | ||
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* '''Dec-01 Tu''' - [ | * '''Dec-01 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]] | ||
:::: [[Image:AlgaeEurope.jpg|60px|AlgaeEurope]] <big>'''[[AlgaEurope 2020 Rome IT]]'''</big> | :::: [[Image:AlgaeEurope.jpg|60px|AlgaeEurope]] <big>'''[[AlgaEurope 2020 Rome IT]]'''</big> | ||
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* '''Dec-15 Tu''' - [ | * '''Dec-15 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]] | ||
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Revision as of 13:40, 5 October 2020
2020 Week 42
- Oct-12 Mo http://www.metabesity2020.com/
If using the Amplex UltraRed assay, when should the background H2O2 calibration be performed? Background H2O2 calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H2O2 is prepared, or if a different respiration medium is used. As the H2O2 stock solution needs to be freshly prepared, it is recommended to perform a background H2O2 calibration before each experiment and at a minimum once per experimental day.
- »How to find the background H2O2 calibration protocol in DatLab « select “AmR calibration” for the background H2O2 calibration protocol
- communicated by »Komlodi Timea« and »Schmitt Sabine«
- Oct-13 Tu
- Oct-14 We
- Oct-15 Th
- Oct-16 Fr
- Oct-18 Su
2020 Week 43
- Oct-19 Mo
Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?
Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.
- You can read more about it in the following publication: »Krumschnabel 2014 Methods Enzymol«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«
- Oct-20 Tu - ÖGMBT Life Science Tuesdays
- Oct-21 We
- Oct-22 Th
- Oct-23 Fr
2020 Week 44
- Oct-26 Mo
- Oct-27 Tu
- Oct-28 We
- Oct-29 Th
- Oct-30 Fr
2020 Week 45
- Nov-02 Mo
- Nov-03 Tu - ÖGMBT Life Science Tuesdays
- Nov-04 We
- Nov-05 Th - MitoEAGLE Summit Obergurgl 2020
- Nov-06 Fr - MitoEAGLE Summit Obergurgl 2020
- Registration deadline for: Integrative Physiology of Exercise 2020 Virtual Event
- Nov-07 Sa - MitoEAGLE Summit Obergurgl 2020
2020 Week 46
- Nov-09 Mo
2020 Week 47
- Nov-16 Mo
- Nov-17 Tu - ÖGMBT Life Science Tuesdays
- Nov-18 We
- Nov-19 Th
- Nov-20 Fr
2020 Week 48
- Nov-23 Mo
- Nov-24 Tu
- Nov-25 We
- » 2020Spring PaduaMuscleDays Padova IT / IOC Padua IT - separate registration
- Nov-26 Th
- Nov-27 Fr
- Nov-28 Sa
2020 Week 49
- Nov-30 Mo
- Dec-01 Tu - ÖGMBT Life Science Tuesdays
- Dec-02 We
- Dec-03 Th
- Dec-04 Fr
- Dec-05 Sa
- Dec-06 So
- Registration deadline - »ESSR 2020 Innsbruck AT«
2020 Week 50
- Dec-07 Mo
- Dec-08 Tu
- Dec-09 We
- ESSR 2020 Innsbruck AT (Dec 9-11)
- Dec-10 Th
- Dec-11 Fr
2020 Week 51
- Dec-14 Mo
- Dec-15 Tu - ÖGMBT Life Science Tuesdays
- Dec-16 We
- Dec-17 Th
- Dec-18 Fr
2020 Week 52
- Dec-21 Mo
- Dec-22 Tu
- Dec-23 We
- Dec-24 Th
- Dec-25 Fr
2020 Week 53
- Dec-28 Mo
- Dec-29 Tu
- Dec-30 We
- Dec-31 Th
- Jan-01 Fr