Template:AmR assay: Difference between revisions
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== Steps and respiratory states == | == Steps and respiratory states == | ||
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| [[DTPA]] | | 0[[DTPA]] | ||
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| [[Superoxide dismutase|SOD]] | | 0[[Superoxide dismutase|SOD]] | ||
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| 0[[Horseradish peroxidase|HRP]] | |||
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| 0[[Amplex UltraRed|AmR]] | |||
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| [[Amplex UltraRed|AmR]] | |||
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Revision as of 12:56, 23 January 2019
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
0DTPA |
DTPA is an iron chelator, which decreases the chemical fluorescence background created by the Amplex UltraRed assay. Administration of DTPA into the O2k-chamber is recommended before all other chemicals because the iron chelation capacity of the compound is time-dependent (approx. 10-15 min). However, the experiments can be carried out in the absence of DTPA. | |||
0SOD |
SOD or superoxide dismutase converts the anion superoxide released by the mitochondria into H2O2, making it accessible to the Amplex UltraRed assay. | |||
0HRP |
HRP or horseradish peroxidase catalyses the conversion of Amplex UltraRed and H2O2 towards the fluorescent resorufin. | |||
0AmR |
AmR or Amplex UltraRed forms resorufin with H2O2 catalysed by horseradish peroxidase HRP. |