Talk:O2k-Open Support alert: Difference between revisions
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::::::: After switching on the O2k, oxygen concentration values might be atypical or different between chambers if air calibration is not performed - Β»[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]Β« | ::::::: After switching on the O2k, oxygen concentration values might be atypical or different between chambers if air calibration is not performed - Β»[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]Β« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] ''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?''' | ||
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - Β»[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]Β« | ::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - Β»[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]Β« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O<sub>2</sub> regime?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O<sub>2</sub> regime?''' |
Revision as of 12:11, 2 April 2020
While in home office mode
- Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
- Why are the oxygen levels different between chambers prior to my experimental run?
- After switching on the O2k, oxygen concentration values might be atypical or different between chambers if air calibration is not performed - Β» Oxygen calibration - troubleshootingΒ«
- Why are the oxygen levels different between chambers prior to my experimental run?
- Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?
- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - Β» Pyruvate - troubleshootingΒ«
- Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O2 regime?
- Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?
- To obtain an accurate O2 background correction, you must select the marks that correspond to your range of experimental O2 levels. - Β» O2 Background correction - troubleshootingΒ«
Removed for correction
- Do you perform the leaky chamber test after chamber assembly?
- - An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly. - Β»Leaky chamber testΒ«
- Do you perform the leaky chamber test after chamber assembly?
In discussion
- Do you know how the "switch components" approach can guide you to find what is causing your O2k not to reach the required specifications?
- Do not desperate if specifications given in the O2k-Manuals are not obtained. See how to perform an experimental troubleshooting on your own.-Β»Switch components approachΒ«
- Do you know how the "switch components" approach can guide you to find what is causing your O2k not to reach the required specifications?
- Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
- See how to clean and dry the electrical connections of the OroboPOS-Connector.-Β»Cleaning the electrical connectionsΒ«
- Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - Β»O2k-OroboPOS-HolderΒ«
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- New Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - Β» Oxygen calibration - DatLabΒ«
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - Β»O2k-Chamber MitoPediaΒ«
- Do you use the correct O2 solubility factor (FM) in the βO2 calibrationβ window in DatLab?
- New Calibration parameters are transferred to experimental DatLab files
- The O2 solubility factor varies according to the medium used and influences the air calibration. - Β»Oxygen solubility factorΒ«