Talk:O2k-Open Support alert: Difference between revisions

Revision as of 09:57, 5 March 2020

Do you perform the leaky chamber test after chamber assembly?

After chamber reassembly you might experience a small leaky chamber only identified by a leaky chamber test- »Leaky chamber test«

Do you change the slope smoothing to 20 while running experiments with biological sample?

While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »Slope smoothing«

Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O₂ background on the O2k-sV-Module?

By adjusting the dithionite concentration to 2.5 mM, the instrumental O₂ background can be performed with specific settings for the O2k-sV-Module. - »O2k-sV-Module«

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 ::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«
-:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Did you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module|Small O2k-Chamber]] (0.5 mL)?'''
+:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module]]?'''
-::::::: By adjusting the dithionite concentration to  2.5 mM, instrumental O2 background can be performed with the standard settings on the [[O2k-sV-Module|Small O2k-Chamber]] (0.5 mL).  ''Further details in'' [[Dithionite]] and »[[MiPNet14.06 Instrumental O2 background]]
+::::::: By adjusting the [[Dithionite|dithionite]] concentration to  2.5 mM, the [[MiPNet14.06 Instrumental O2 background|instrumental O<sub>2</sub> background]] can be performed with specific settings for the [[O2k-sV-Module]]. - »[[O2k-sV-Module]]«