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Difference between revisions of "Talk:MitoFit Proficiency Test"

From Bioblast
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| 2018-10-15
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| DatLab Files have to be sent to [email protected]
| DatLab Files have to be sent to [[mailto:[email protected]|[email protected]]]
Shipping of HEK cells
Shipping of HEK cells
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Revision as of 13:23, 3 October 2018

Important information

How much time is needed for the execution of the test?

Level 1. Air calibration and Instrumental background oxygen flux: ca. 3 hours
Level 2. SUIT-1 or SUIT-2: ca. 3 hours, including air calibration
Level 3. Data analysis: 1 hour
Within the timespan of one workday, your personal proficiency is comprehensively evaluated, using your own O2k and your laboratory equipment

Is there any scientific objective?

Together with a literature review and preliminary data from the Oroboros MitoFit laboratory, we are going to publish the test results in collaboration with the Department for Medical Statistics, Informatics and Health Economics, Medical University of Innsbruck, as part of the K-Regio Project MitoFit.
With the participation in the MitoFit Proficiency Test, you agree on the use of experimental data collected for the test, to be used for research purposes in an anonym way.

Additional resources:

For a comprehensive list of DatLab menus: MitoPedia DatLab
MitoPedia - SUIT
Flux analysis
DatLab Protocols, DLP: List of titrations, marks, overview on the website.


Time schedule

Round 01

Date
2018-10-09 DatLab 7.3 and instructions to be sent to the participants of the study
2018-10-10 to 15 Level IPT to be performed: instrumental background oxygen flux and air calibration, using the Instrumental DLPs
2018-10-15 DatLab Files have to be sent to [[1]]

Shipping of HEK cells

2018-10-17 Arrival of shipment at customerโ€™s site at the latest

Feedback on Level I sent to participants

2018-10-17 to 26 Level II PT to be performed: SUIT 1 / 2 technical repeats
2018-10-28 DatLab files to be sent to Oroboros for analysis (Level II)
2018-10-31 Feedback to the participants