Search results

• SUIT-031
  ...are designed to measure simultaneously O₂ flux and the Q-redox state in isolated mitochondria and permeabilized cells. ...t D072]] simultaneous determination of O₂ flux and the Q-redox state in isolated mitochondria and cells permeabilized previously to addition to
  4 KB (623 words) - 17:57, 13 December 2023
• SUIT-039 O2 mt D092
  ...er electron transfer pathways are also analyzed in OXPHOS (FN, FNS) and ET-state (FNS, S). :::+ The protocol provides information on F-pathway in OXPHOS state with palmitoylcarnitine, a long-chain acylcarnitine commonly found ''in viv
  3 KB (387 words) - 11:21, 14 April 2023
• SUIT-039 O2 pfi D093
  ...er electron transfer pathways are also analyzed in OXPHOS (FN, FNS) and ET-state (FNS, S). :::+ The protocol provides information on F-pathway in OXPHOS state with palmitoylcarnitine, a long-chain acylcarnitine commonly found ''in viv
  3 KB (387 words) - 12:30, 14 April 2023
• Schinagl 2016 PLoS One
  ...r W (2016) Adapting high-resolution respirometry to glucose-limited steady state mycelium of the filamentous fungus ''Penicillium ochrochloron'': method dev ...lium and a direct transfer of a broth sample into the respirometer. Steady state respiration was 29% below its maximum respiratory capacity. Additionally to
  3 KB (386 words) - 15:46, 14 October 2023
• Cardoso 2022a EBEC
  ...of the Q-junction, respectively, impacts the relation between the Q redox state and O₂ flux in opposite directions. Therefore, our aim was to ch ...he NextGen-O2k with Q-Module to measure O₂ flux and the Q redox state simultaneously, using CoQ₂ as a probe [3]. Cryopreserved HEK 293
  4 KB (519 words) - 14:25, 23 August 2022
• SUIT-021 O2 mt D035
  ...tate| S]] and [[NADH Electron transfer-pathway state| N-]] pathway control state. ...s to be determined. Higher concentrations of oligomycin may inhibit the ET state.
  3 KB (420 words) - 09:20, 18 September 2020
• Komlodi 2018 AussieMit
  ...O₂ flux, H₂O₂ flux, and coenzyme Q redox state. AussieMit 2018 Melbourne AU. ...an altered Q redox state. We analyzed the relationship between the Q redox state, H₂O₂ and O₂ fluxes with fuel substrates p
  3 KB (517 words) - 18:56, 10 January 2022
• Verkhovsky 1996 Nature
  ...coli leads to a corresponding decrease in the enzyme's operational steady-state affinity for O2. This demonstrates directly that fast electron transfer bet
  2 KB (247 words) - 21:39, 24 September 2021
• Komlodi 2021 MitoFit Q
  ...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/mitofit: ...omlodi_2021_MitoFit_Q.pdf Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]
  5 KB (767 words) - 08:41, 8 January 2023
• Cardoso 2022 ESCI Bari
  ...getic fingerprinting with coupling and pathway control of coenzyme Q redox state and respiration in permeabilized HEK 293T cells. 56^th Annual Sci We characterised the Q redox state in pathway and coupling control using the Oroboros NextGen-O2k with the ele
  4 KB (520 words) - 14:41, 30 May 2022
• SUIT-028
  ! Respiratory state ...ate with type N substrates, N_''L'': Non-phosphorylating resting state with NADH-linked (type N) substrates pyruvate&glutamate&malate (PGM; withou
  4 KB (574 words) - 16:13, 14 April 2020
• SUIT-021
  ...support NADH-linked respiration. Oligomycin (Omy) is used to induce a LEAK state of respiration via the inhibition of the ATP synthase. Since higher concent ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.
  5 KB (697 words) - 15:58, 8 June 2020
• Succinate pathway
  ...that supports succinate-linked respiration (succinate-induced respiratory state; previously used nomenclature: CII-linked respiration; SRot; see [[Gnaiger The S-pathway control state is usually induced in mt-preparations by addition of succinate&rotenone. In
  8 KB (1,053 words) - 08:04, 30 March 2023
• Electron-transfer-pathway state
  |abbr=''ET-pathway state'' ...state]]s. [[Coupling-control state]]s require [[Electron-transfer-pathway state|ET-pathway competent states]], including oxygen supply. [[Categories of SUI
  15 KB (1,913 words) - 19:27, 25 August 2023
• SUIT-020
  ...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.
  5 KB (756 words) - 15:03, 17 September 2020
• SUIT-021 Fluo mt D036
  ...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.
  6 KB (834 words) - 14:07, 6 September 2021
• State 3
  ...ate]] of respiration, whereas the cytochromes are in a more oxidized redox state. |mitopedia concept=Respiratory state, Find
  3 KB (449 words) - 18:46, 1 January 2021
• Chicco 2022 Abstract Bioblast
  ...(NS > S). However, in some types of mitochondria, rotenone added in the NS-state elicits a paradoxical increase in ''J''_O₂, revealing ::::# Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA - [email protected]
  4 KB (625 words) - 08:33, 28 July 2022
• SUIT-009 AmR mt D021
  ...in the LEAK state. Pyruvate (P) supports [[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP it does not increase ...s protocol is designed to evaluate RET in the S-linked pathway in the LEAK state, which is decreased by ADP due to reduction of the mt-membrane potential.
  5 KB (681 words) - 00:11, 13 April 2021
• Komlodi 2022 Q10 Hamburg
  ...s: Continuous monitoring of pull and push control of respiration and redox state of the Q-mimetic CoQ₂. Q10 Hamburg. ...mplemented into the Oroboros NextGen-O2k to monitor continuously the redox state of CoQ2 added as a Q-mimetic simultaneously with O2 consumption (Komlódi e
  4 KB (559 words) - 18:39, 5 May 2022

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