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• O2k-chamber
  |description=[[File:O2k-Chamber.jpg|right|180px]] ...with standard operation volumes (''V'') of 2.0 mL or 0.5 mL (small chamber volume in the [[O2k-sV-Module]], 12 mm inner diameter). The optical properties of
  12 KB (1,709 words) - 13:03, 5 February 2024
• NextGen-O2k Documentation
  ...www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]<br> ....com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
  5 KB (760 words) - 13:23, 3 April 2022
• Baglivo 2022 BEC
  ...>O₂</sub> in the absence of sample. Finally, zero O₂ calibration completes the sensor test. ...ly intervals. These results confirm the instrumental limit of detection of volume-specific O₂ flux at ±1 pmol∙s⁻¹∙mL<sup>−1</s
  4 KB (588 words) - 04:54, 9 March 2023
• Rhodamine 123
  ...L aliquot 5 mM storage solution with 2400 µL ethanol to reach 2.5 mL final volume; ::* Titration volume: in 2-µL steps using a 10 µL syringe (2 mL O2k-Chamber).
  5 KB (698 words) - 15:50, 16 March 2021
• Baglivo 2022 MitoFit-QC
  ...>O₂</sub> in the absence of sample. Finally, zero O₂ calibration completes the sensor test. ...ly intervals. These results confirm the instrumental limit of detection of volume-specific O₂ flux at ±1 pmol∙s⁻¹∙mL<sup>−1</s
  5 KB (653 words) - 09:12, 27 March 2024
• Safranin
  ...3.508 mg of Safranin and dissolve in distilled water to reach 10 mL final volume; ::::* Titration volume: in 1µL steps using a 10 µL syringe (2 mL O2k-chamber).
  13 KB (1,842 words) - 17:09, 17 January 2024
• Select plots - DatLab
  ...ignal can be changed, Flux/Slope can be chosen to be displayed as Flux per volume or as normalized specific flux/flow, the background correction can be switc ...two sections are for O2k chamber A, the two section on the bottom for O2k chamber B, in each case the first row is for the signal, the second for Flux/Slope.
  5 KB (769 words) - 15:28, 11 April 2018
• O2k-Virtual Support
  ::1. O2k-chamber assembly ::4. Volume calibration
  6 KB (752 words) - 12:53, 7 November 2023
• Oxygen calibration - DatLab
  ...gh-resolution respirometry with the calibration routine using instrumental calibration DL-Protocols in [[DatLab]]. |info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]
  26 KB (3,995 words) - 10:15, 7 October 2021
• Baglivo 2022b ESCI Bari
  ...>/dt to determine oxygen consumption by the POS and backdiffusion into the chamber [1]. We evaluated instrumental tests of 48 Oroboros O2k chambers obtained f Stability of oxygen calibration signals at air saturation and zero oxygen was monitored up to 8 months. The
  3 KB (419 words) - 15:24, 30 May 2022
• MiPNet14.13 Medium-MiR06
  * Total volume of [[solution]] = 1 litre. ::7) Add H₂0 to a final volume of 1000 mL.
  8 KB (1,272 words) - 10:25, 16 March 2022
• Oxygen solubility
  |info=[[MiPNet06.03 POS-calibration-SOP]] ...implemented in the oxygen calibration algorithms in HRR ([[MiPNet06.03 POS-calibration-SOP]]).
  12 KB (1,907 words) - 10:24, 27 October 2022
• Dithionite
  ...r [[Oxygen calibration - DatLab |calibration of oxygen sensors]] at [[Zero calibration | zero oxygen concentration]], or for stepwise reduction of oxygen [[concen |info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet14.06 Instrumental O2 background]]
  9 KB (1,336 words) - 07:33, 13 October 2023
• Cell count and normalization in HRR
  ...|right|link=Tube Racks|thumb|'''Figure 1.''' Stoppers removed from the O2k-chamber are placed into dry Falcon tubes on the Tube Rack preventing contamination. ...nical details of the procedures used when adding cell suspensions into the chamber with stopper off and removed ('''Figure 1'''), as summarized in the followi
  35 KB (5,607 words) - 16:01, 21 February 2024
• TMRM
  ...aliquot of the 5 mM storage solution with 800 µL DMSO to reach 1 mL final volume; ...aliquot of the 5 mM storage solution with 960 µL DMSO to reach 1 mL final volume;
  17 KB (2,405 words) - 07:29, 5 April 2024
• Proficiency test
  ...on, enter O₂ solubility factor; use DatLab-Excel template '''O2-calibration.xlsx'''. ::::# O2k quality control 2: evaluate O2k chamber test; DatLab analysis of a file obtained in an instrumental O₂ b
  6 KB (767 words) - 16:54, 29 August 2022
• O2k-OpenSupportAlert2020 Archive
  Yes, oxygen signals obtained after proper calibration are independent of the gain setting, and can therefore be grouped together ...ex UltraRed]] assay, when should the background H₂O₂ calibration be performed?
  27 KB (4,026 words) - 11:53, 29 March 2021
• MiPNet25.02 IOC Schroecken AT
  ...when receiving support on instrumental setup, including O2k chamber-volume calibration and service of the OroboPOS.
  3 KB (388 words) - 08:30, 21 October 2020
• Flux / Slope
  ...is the name of the pull-down menu for (1) normalization of flux (chamber volume-specific flux, sample-specific flux or flow, or flux control ratios), (2) [ ...be sure to use the standard Layout 04a (Flux per volume) or 04b (Flux per volume overlay). When starting with the instrumental standard Layouts 1-3, which d
  17 KB (2,751 words) - 09:55, 17 November 2023
• Oxygen flux - instrumental background
  ...POS]], and oxygen diffusion into or out of the aqueous medium in the [[O2k-chamber]]. It is a property of the instrumental system, measured in the range of ex == O₂ slope in the closed and open O2k-chamber ==
  22 KB (3,238 words) - 15:29, 2 May 2024

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