SUIT reference protocol: Difference between revisions
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|description=The [[SUIT reference assay]] consists of two '''SUIT reference protocols''' ([[SUIT-RP1]] and [[SUIT-RP2]]) which are [[harmonized SUIT protocols]]. These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis. | |description=The [[SUIT reference assay]] consists of two '''SUIT reference protocols''' ([[SUIT-RP1]] and [[SUIT-RP2]]) which are [[harmonized SUIT protocols]]. These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis. | ||
: SUIT-RP1: [[PM(LPcE)+Oct+G+S+Rot+Gp+Ama+Tm+Azd]] | |||
: SUIT-RP1: [[1PM(L2Pc3E)_4Oct_5G_6S_7Rot_8Gp_9Ama_10Tm_11Azd]] | |||
: SUIT-RP2: [[D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd]] | |||
: SUIT-RP2: [[1D(ROX)_2Oct(P)_3M_4Pc_5G_6S(P7E)_8Gp_9Rot_10Ama_11Tm_12Azd]] | |||
|info=[[MiPNet21.06 SUIT reference assay]] | |info=[[MiPNet21.06 SUIT reference assay]] | ||
}} | }} |
Revision as of 00:50, 26 January 2016
Description
The SUIT reference assay consists of two SUIT reference protocols (SUIT-RP1 and SUIT-RP2) which are harmonized SUIT protocols. These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.
- SUIT-RP1: PM(LPcE)+Oct+G+S+Rot+Gp+Ama+Tm+Azd
- SUIT-RP1: 1PM(L2Pc3E)_4Oct_5G_6S_7Rot_8Gp_9Ama_10Tm_11Azd
- SUIT-RP2: D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd
- SUIT-RP2: 1D(ROX)_2Oct(P)_3M_4Pc_5G_6S(P7E)_8Gp_9Rot_10Ama_11Tm_12Azd
Abbreviation: SUIT-RP
Reference: MiPNet21.06 SUIT reference assay
Cross-linked respiratory states
The cross-linked respiratory states for harmonization between protocols are: