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Revision as of 18:20, 24 January 2019 by Komlodi Timea (talk | contribs) (Created page with "{{MitoPedia |abbr=NS(GM) |description=300px|SUIT-018 |info='''A''': '''SUIT-018''' to study the oxygen dependence of H<sub>2</sub>O<sub>...")
- high-resolution terminology - matching measurements at high-resolution
SUIT-018 AmR mt D031
Description
Abbreviation: NS(GM)
Reference: A: SUIT-018 to study the oxygen dependence of H2O2 (AmR) in mitochondrial preparations (isolated mitochondria and tissue homogenate).
SUIT number: D031_1GMS;2D;3Ama
O2k-Application: AmR
- SUIT-category: NS(GM)
- SUIT protocol pattern: linear 1GMS;2D;3Ama
Communicated by Iglesias-Gonzalez J, Komlodi T and Gnaiger E (last update 2019-01-22)
DLP applications
References
| Year | Reference | Organism | Tissue;cell | |
|---|---|---|---|---|
| MiPNet24.10 H2O2 flux analysis | 2021-10-22 |  | ||
| Komlodi 2021 BEC AmR-O2 | 2021 | Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004 | Saccharomyces cerevisiae | Other cell lines |
| MiPNet20.14 AmplexRed H2O2-production | 2019-06-24 | | Mouse | Heart |
| Komlodi 2018 Methods Mol Biol | 2018 | Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. | Human Mouse | Skeletal muscle HEK |
| Makrecka-Kuka 2015 Biomolecules | 2015 | Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 | Human Mouse | Heart Nervous system HEK |
| Krumschnabel 2015 Methods Mol Biol | 2015 | Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. | Mouse | Nervous system |
Strengths and limitations
- + Reasonable duration of the experiment.
- + Simple protocol to evaluate the oxygen dependence of H2O2 production.
- - Measurements with Amplex UltraRed assay cannot be carried out with liver homogenate.
- - H2O2 production can be studied in two different states, LEAK and OXPHOS.
- - Reoxygenation and nitrogen injection could lead to bubble formation in the O2k-Chamber
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Fluorometry
