Difference between revisions of "SUIT-009 O2 ce-pce D016"

Revision as of 15:15, 21 January 2019

high-resolution terminology - matching measurements at high-resolution

SUIT-009 O2 ce-pce D016

Abbreviation: NS(P)

Reference: A: SUIT-009 short protocol for mt-respiration in pce.

SUIT-category: NS(P)

SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot;5Ama

For H2O2-Application, choose SUIT-009 AmR pce D019; for O2 and H₂O₂ measurements (AmR), choose: SUIT-009 AmR pce D019.
Cytochrome C test can be performed in the S-OXPHOS state.
Reasonable duration of the experiment.

- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.

MitoPedia concepts: SUIT protocol, SUIT A, Find

MitoPedia methods: Respirometry

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=NS(P)
-|description=[[File:1S;2D;3P;4Rot-.png|400px|SUIT-009]]
+|description=[[File:1S;2D;3P;4Rot-.png|300px|SUIT-009]]
 |info='''A''': '''[[SUIT-009]]''' short protocol for mt-respiration  in [[permeabilized cells |pce]].
-|application=O2
-|SUIT number=D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama
 }}
 ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(P)
-::: '''[[SUIT protocol pattern]]:''' orthogonal 1S;2D;3P;4Rot
+::: '''[[SUIT protocol pattern]]:''' orthogonal 1S;2D;3P;4Rot;5Ama
 __TOC__
-{{MitoPedia concepts
-|mitopedia concept=SUIT protocol, SUIT A, Find
-}}
-{{MitoPedia methods
-|mitopedia method=Fluorometry
-}}
 == Strengths and limitations ==
@@ Line 23: / Line 15: @@
 :::* Reasonable duration of the experiment.
 :::- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.
+{{MitoPedia concepts
+|mitopedia concept=SUIT protocol, SUIT A, Find
+}}
+{{MitoPedia methods
+|mitopedia method=Respirometry
+}}