Difference between revisions of "SUIT-009 O2 ce-pce D016"

Revision as of 13:28, 21 January 2019

high-resolution terminology - matching measurements at high-resolution

SUIT-009 O2 ce-pce D016

Abbreviation: NS(P)

Reference: A: SUIT-009 short protocol for mt-respiration in pce.

SUIT number: D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama

O2k-Application: O2

SUIT-category: NS(P)

SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot

MitoPedia concepts: SUIT protocol, SUIT A, Find

MitoPedia methods: Fluorometry

For H2O2-Application, choose SUIT-009 AmR pce D019; for O2 and H₂O₂ measurements (AmR), choose: SUIT-009 AmR pce D019.
Cytochrome C test can be performed in the S-OXPHOS state.
Reasonable duration of the experiment.

- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.

@@ Line 10: / Line 10: @@
 __TOC__
-== 1S;2D;3P;4Rot;5Ama-==
-SUIT-009 is a short protocol for determination of O<sub>2</sub> flux  on permeabilized cells. Succinate (S) supports the reverse electron transfer (RET) initiated H<sub>2</sub>O<sub>2</sub> production in the LEAK state. Addition of ADP increases S-OXPHOS capacity. Pyruvate (P) supports [[NADH Electron transfer-pathway state|NADH-pathway (N)]]. Antimycin A (Ama) inhibits Complex III (CIII) and results in the inhibition of O<sub>2</sub> flux.
-For H2O2-Application, choose [[SUIT-009 AmR pce D019]]; for O2 and H<sub>2</sub>O<sub>2</sub> measurements (AmR), choose: [[SUIT-009 AmR pce D019]].
 {{MitoPedia concepts
@@ Line 23: / Line 19: @@
 == Strengths and limitations ==
+:::*For H2O2-Application, choose [[SUIT-009 AmR pce D019]]; for O2 and H<sub>2</sub>O<sub>2</sub> measurements (AmR), choose: [[SUIT-009 AmR pce D019]].
 :::* Cytochrome C test can be performed in the S-OXPHOS state.
 :::* Reasonable duration of the experiment.
 :::- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.