Difference between revisions of "SUIT-009 O2 ce-pce D016"
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== Strengths and limitations == | |||
:::* Cytochrome C test can be performed in the S-OXPHOS state. | |||
:::* Reasonable duration of the experiment. | |||
:::- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway. | |||
Revision as of 13:21, 21 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-009 O2 ce-pce D016
Description
Abbreviation: NS(P)
Reference: A: SUIT-009 short protocol for mt-respiration in pce.
SUIT number: D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama
O2k-Application: O2
- SUIT-category: NS(P)
- SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot
1S;2D;3P;4Rot;5Ama-
SUIT-009 is a short protocol for determination of O2 flux on permeabilized cells. Succinate (S) supports the reverse electron transfer (RET) initiated H2O2 production in the LEAK state. Addition of ADP increases S-OXPHOS capacity. Pyruvate (P) supports NADH-pathway (N). Antimycin A (Ama) inhibits Complex III (CIII) and results in the inhibition of O2 flux. For H2O2-Application, choose SUIT-009 AmR pce D019; for O2 and H2O2 measurements (AmR), choose: SUIT-009 AmR pce D019.
MitoPedia concepts:
SUIT protocol,
SUIT A,
Find
MitoPedia methods:
Fluorometry
Strengths and limitations
- Cytochrome C test can be performed in the S-OXPHOS state.
- Reasonable duration of the experiment.
- - In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.
