From Bioblast
Revision as of 17:23, 8 February 2019 by Cardoso Luiza (talk | contribs) (Created page with "{{MitoPedia |abbr=N(PM) |description=300px |info='''A''' |application=O2 |SUIT number=D047_1PM;2D;2c;3Omy;4U;5Ama }} ::: '''Categories of...")
- high-resolution terminology - matching measurements at high-resolution
SUIT-006 O2 mt D047
Description
Abbreviation: N(PM)
Reference: A
SUIT number: D047_1PM;2D;2c;3Omy;4U;5Ama
O2k-Application: O2
- SUIT-category: N(PM)
- SUIT protocol pattern: Bidirectional linear coupling control protocol 1PM;2D;2c;3Omy;4U;5Ama
References
Strengths and limitations
- This protocol is indicated when the aim is to analyse coupling control in isolated mitochondria or tissue homogenates at N(PM) electron transfer-pathway state.
- + Short duration of the experiment.
- + CIV activity can be measured as an extension.
- - This protocol analyses coupling control at N-pathway, however, it is less specific than a similar protocol containing the complex II inhibitor malonic acid, that would allow to analyse the N-pathway without any contribution of the S-pathway.
- - This protocol does not include internal ET-pathway control steps.
- - Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors, if used.
Compare SUIT protocols
- SUIT-001 O2 mt D001: Extended protocol with SUIT-006 without oligomycin as the initial coupling control module, for mitochondrial preparations.
- SUIT-003: Coupling control protocol for non-permeabilized cells (ce), counterpart to SUIT-006 for mt-preparations (mtprep).
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry
