Ruthenium red: Difference between revisions
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}}<!--:::'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>);ย Sigma R 2751, 1 g; M = 786.35 gยทmol<sup>-1</sup>.--> | }}<!--:::'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>);ย Sigma R 2751, 1 g; M = 786.35 gยทmol<sup>-1</sup>.--> |
Revision as of 12:05, 10 June 2021
Description
Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca2+ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca2+-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].
Abbreviation: RR
Reference: MiPNet03.02, Wikipedia
Application in HRR
- Rut: Ruthenium red (Ammoniated ruthenium oxychloride; (NH3)5RuORu(NH3)4ORu(NH3)5Cl6), Sigma-Aldrich: R2751, store at RT, CAS: 11103-72-3, M = 786.35 gยทmol-1
- Hazard statements: H302; Harmful if swallowed
- Rut: Ruthenium red (Ammoniated ruthenium oxychloride; (NH3)5RuORu(NH3)4ORu(NH3)5Cl6), Sigma-Aldrich: R2751, store at RT, CAS: 11103-72-3, M = 786.35 gยทmol-1
- Preparation of 2 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 1.57 mg Ruthenium Red.
- Dissolve in 1 mL H2O.
- Divide into 0.1 mL aliquots.
- Store at -20 ยฐC.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 1 ยตM.
- Preparation of 0.5 mM stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 50 ยตL of the 1 mM stock solution.
- Add 150 ยตL H2O.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 1 ยตM.
References
- Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
- Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
MitoPedia topics:
Inhibitor