Difference between revisions of "Rotenone"

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Rotenone

Description

Rotenone is an (synonym. 2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on) inhibitor of complex I via inhibition of NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S_(Rot).

Abbreviation: Rot

Reference: Gnaiger 2012 MitoPathways, MiPNet09.12, MiPNet03.02

MitoPedia topics: Inhibitor

Application in HRR

Rot: Rotenone (Rotenone ≥ 95%, C₂₃H₂₂O₆); Sigma R 8875, 1 g, store at R.T.; FW = 394.42

Caution: Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!

Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 0.39 mg of Rotenone and dissolve in 1 ml of ethanol absolute (99.9%)

2) divide into 100 µl aliquots (use dark glass vials)

3) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 1 µl using a 10 µl syringe
Final conc. in 2 ml O2k-chamber: 0.5 µM

@@ Line 2: / Line 2: @@
 |abbr=Rot
 |description='''Rotenone''' is an (synonym. 2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on) inhibitor of [[complex I]] via inhibition of NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also [[S(Rot)|S<sub>(Rot)</sub>]].
-|info=[[MiPNet11.09]], [[MiPNet09.12]], [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet03.02]]
+|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet09.12]], [[MiPNet03.02]]
 |type=Inhibitor ETS
 }}