Difference between revisions of "Rauchova 2005 Biochem Biophys Res Comm"
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{{Publication | {{Publication | ||
|title=Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone. Biochem | |title=Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone. Biochem Biophys Res Comm 339: 362-366. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/16300743 PMID: 16300743] | |||
|authors=Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z | |authors=Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z | ||
|year=2005 | |year=2005 | ||
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|abstract=The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs—idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ<sub>1</sub> were found to be more efficient in the scavenging activity (IC<sub>50</sub> : 0.052 and 0.075 μM, respectively) than CoQ<sub>3</sub> (IC<sub>50</sub> : 45.8 μM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation | |abstract=The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs—idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ<sub>1</sub> were found to be more efficient in the scavenging activity (IC<sub>50</sub> : 0.052 and 0.075 μM, respectively) than CoQ<sub>3</sub> (IC<sub>50</sub> : 45.8 μM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation | ||
|keywords=Mitochondrial glycerophosphate dehydrogenase, Succinate dehydrogenase, Reactive oxygen species, debenone, Coenzyme Q analogs | |keywords=Mitochondrial glycerophosphate dehydrogenase, Succinate dehydrogenase, Reactive oxygen species, debenone, Coenzyme Q analogs | ||
| | |discipline=Pharmacology; Biotechnology | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |instruments=Oxygraph-2k | ||
|enzymes=Complex II; Succinate Dehydrogenase, Complex III | |enzymes=Complex II; Succinate Dehydrogenase, Complex III | ||
|topics=Respiration; OXPHOS; ETS Capacity, Flux Control; Additivity; Threshold; Excess Capacity | |topics=Respiration; OXPHOS; ETS Capacity, Flux Control; Additivity; Threshold; Excess Capacity | ||
| | |discipline=Pharmacology; Biotechnology | ||
}} | }} | ||
Revision as of 23:01, 12 December 2011
| Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone. Biochem Biophys Res Comm 339: 362-366. |
Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Biochem. Biophys. Res. Commun.
Abstract: The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs—idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ1 were found to be more efficient in the scavenging activity (IC50 : 0.052 and 0.075 μM, respectively) than CoQ3 (IC50 : 45.8 μM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation • Keywords: Mitochondrial glycerophosphate dehydrogenase, Succinate dehydrogenase, Reactive oxygen species, debenone, Coenzyme Q analogs
Labels:
Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property., Complex III Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Flux Control; Additivity; Threshold; Excess Capacity"Flux Control; Additivity; Threshold; Excess Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
