Rauchova 2005 Biochem Biophys Res Comm
|Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone. Biochem Biophys Res Comm 339:362-6.|
Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Biochem. Biophys. Res. Commun.
Abstract: The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs—idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ1 were found to be more efficient in the scavenging activity (IC50 : 0.052 and 0.075 μM, respectively) than CoQ3 (IC50 : 45.8 μM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation • Keywords: Mitochondrial glycerophosphate dehydrogenase, Succinate dehydrogenase, Reactive oxygen species, debenone, Coenzyme Q analogs
• O2k-Network Lab: CZ Prague Houstek J, IT Bologna Lenaz G, IT Bologna Genova ML
Labels: MiParea: Respiration
Pathway: S, Gp, Other combinations HRR: Oxygraph-2k