From Bioblast
- high-resolution terminology - matching measurements at high-resolution
PH calibration buffers
Description
pH calibration buffers are prepared to obtain two or more defined pH values for calibration of pH electrodes and pH indicator dyes.
Hanks based calibration buffers
- Modified hanks buffer
- CaCl2 * 2 H2O 0.42 mM, imidazole 2.0 mM, potassium chloride 2.4 mM, magnesium chloride hexahydrate 0.49 mM, sodium chloride 137 mM, pH adjusted to 7.1 at 30°C; see also: [1]
- Modified hanks buffer
ph adjusted with KOH, HCl to create a buffer series: (pH values at 25 °C): A(3.3), B(6.1), C(6.9 initial measurement showed 6.4), D (6.9), E(7.3), F(7.5), G(10.1). for experiments after MF613 buffer C was further adjusted to 6.6 and G was adjusted to 11.1 ( MF613 showed that buffer G at 37° was not yet representing the basic endpoint for carboxy-SNARF-1)
