PH calibration buffers: Difference between revisions
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== Hanks based calibration buffers == | == Hanks based calibration buffers == | ||
::: '''Modified hanks buffer''' | ::: '''Modified hanks buffer''' | ||
Revision as of 10:12, 8 September 2018
- high-resolution terminology - matching measurements at high-resolution
PH calibration buffers
Description
pH calibration buffers are prepared to obtain two or more defined pH values for calibration of pH electrodes and pH indicator dyes.
MitoPedia methods: Respirometry, Fluorometry
MitoPedia topics: Media for respirometry
Hanks based calibration buffers
- Modified hanks buffer
- CaCl2 * 2 H2O 0.42 mM, imidazole 2.0 mM, potassium chloride 2.4 mM, magnesium chloride hexahydrate 0.49 mM, sodium chloride 137 mM, pH adjusted to 7.1 at 30°C; see also: [1]
- Modified hanks buffer
ph adjusted with KOH, HCl to create a buffer series: (pH values at 25 °C): A(3.3), B(6.1), C(6.9 initial measurement showed 6.4), D (6.9), E(7.3), F(7.5), G(10.1). for experiments after MF613 buffer C was further adjusted to 6.6 and G was adjusted to 11.1 ( MF613 showed that buffer G at 37° was not yet representing the basic endpoint for carboxy-SNARF-1)
