Difference between revisions of "Oxygen flux - instrumental background"
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{{MitoPedia | {{MitoPedia | ||
|abbr=''J''°<sub>O2</sub> | |abbr=''J''°<sub>O2</sub> | ||
|description='''Instrumental background oxygen flux''', ''J''°<sub>O2</sub>, in a respirometer is due to oxygen consumption by the [[POS]], and oxygen diffusion into or out of the aqueous medium in the [[O2k-Chamber]]. It is measured in the range of experimental oxygen levels by a standardized [[instrumental background test]], and is a property of the instrumental system. | |description='''Instrumental background oxygen flux''', ''J''°<sub>O2</sub>, in a respirometer is due to oxygen consumption by the [[POS]], and oxygen diffusion into or out of the aqueous medium in the [[O2k-Chamber]]. It is measured in the range of experimental oxygen levels by a standardized [[instrumental background test]], and is a property of the instrumental system. Instrumental background correction eliminates errors by systemic flux compensation, automatically performed by [[DatLab]]. | ||
Automatic correction for the instrumental background oxygen flux is an essential standard in high resolution respirometry. At the same time an instrumental background experiment is the ultimate test for instrumental performance, evaluating chamber performance after completion of all elements of the [[Sensor test]]. The instrumental background oxygen flux measured at air saturation should reflect the theoretically predicted volume-specific oxygen consumption by the oxygen sensor. The actual agreement using experimental respiration medium provides at the same time a test that excludes microbial contamination of the medium or serves to evaluate any autoxidation processes in newly tested experimental media. | |||
|info=[[MiPNet14.06]]; [[Gnaiger_2008_POS]]; [[Gnaiger_2001_RespPhysiol]] | |info=[[MiPNet14.06]]; [[Gnaiger_2008_POS]]; [[Gnaiger_2001_RespPhysiol]] | ||
|type=Respiration | |type=Respiration | ||
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{{MitoPedia topics|type=Respiration | {{MitoPedia topics|type=Respiration | ||
}} | }} | ||
* See [http://www.oroboros.at/index.php?id=backgroundcorrection MiPNet14.06] for experimental details of the instrumental background test. The Spreadsheet template for analyzing the Instrumental Background Experiment is available form the same link. | |||
* See also [[Chemical background correction of oxygen flux]]. | |||
{{#set: Technical service=O2 signal|Technical service=Performance test}} | {{#set: Technical service=O2 signal|Technical service=Performance test}} | ||
Revision as of 13:18, 24 November 2012
- high-resolution terminology - matching measurements at high-resolution
Oxygen flux - instrumental background
Description
Instrumental background oxygen flux, J°O2, in a respirometer is due to oxygen consumption by the POS, and oxygen diffusion into or out of the aqueous medium in the O2k-Chamber. It is measured in the range of experimental oxygen levels by a standardized instrumental background test, and is a property of the instrumental system. Instrumental background correction eliminates errors by systemic flux compensation, automatically performed by DatLab.
Automatic correction for the instrumental background oxygen flux is an essential standard in high resolution respirometry. At the same time an instrumental background experiment is the ultimate test for instrumental performance, evaluating chamber performance after completion of all elements of the Sensor test. The instrumental background oxygen flux measured at air saturation should reflect the theoretically predicted volume-specific oxygen consumption by the oxygen sensor. The actual agreement using experimental respiration medium provides at the same time a test that excludes microbial contamination of the medium or serves to evaluate any autoxidation processes in newly tested experimental media.
Abbreviation: J°O2
Reference: MiPNet14.06; Gnaiger_2008_POS; Gnaiger_2001_RespPhysiol
MitoPedia methods:
Respirometry
- See MiPNet14.06 for experimental details of the instrumental background test. The Spreadsheet template for analyzing the Instrumental Background Experiment is available form the same link.
- See also Chemical background correction of oxygen flux.
