O2k-OpenSupportAlert2020 Archive
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
May 2020
- May-11 Th
Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.
- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. - »Steady state«
- May-04 Mo
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Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels? - See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«
Apr 2020
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
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Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases? - DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation - »SUIT-024«
- Apr-08 We
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The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage. - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration. - »SUIT-010«
- Apr-02 Th
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Do you want to search the best SUIT protocol for your research questions? Use the SUITbrowser: »SUITbrowser«
Mar 2020
- Mar-16 Mo
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The standardized coupling control protocol to explore the bioenergetics of living cells - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«
- Mar-11 We
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Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - »Blebbistatin«
- Mar-06 Fr
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Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
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Rapamycin - more differences or more in common with other mitObesity drugs? - »Rapamycin«
Feb 2020
- Feb-26 We
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It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.
- Feb-24 Mo
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More research is needed on the impact of viral infection on mitochondrial function.
- Feb-19 We
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Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - »Spermidine«
- Feb-18 Tu
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Why do we fight gas bubbles? - Dive deep into »Oxygen solubility«
- Feb-12 We
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What do metformin, melatonin and other bioactive mitObesity compounds have in common? - »MitObesity drugs«
Jan 2020
- Jan-16 Th
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Oxygen flux - which SI units should be used?
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Did you ever think that units for body mass could be an issue? - »Body mass«
- Jan-08 We
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Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.
