O2k-OpenSupportAlert2020 Archive: Difference between revisions

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== Apr 2020 ==
== Apr 2020 ==
 
* '''Apr-27 Mo'''
* '''Apr-24 Fr'''  
[[File:ETC-.JPG|right|150px|Electron transport chain]]
:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]].
|'''Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O<sub>2</sub> regime?'''
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«
- To obtain an accurate O<sub>2</sub>  background correction, you must select the marks that correspond to your range of experimental O<sub>2</sub>  levels.
- »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«
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* '''Apr-20 Mo'''
* '''Apr-21 Tu'''  
[[File:CS.png|right|100px|Citrate synthase]]
:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Improve]]
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
|'''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''
- »'''[[MiPNet17.04 CitrateSynthase]]'''«
- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation.
- »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«
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* '''Apr-14 Mo'''
* '''Apr-13 Mo'''
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?'''
|'''Why are the oxygen levels different between chambers prior to my experimental run?'''
- DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]] - »'''[[SUIT-024 |SUIT-024]]'''«
- After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations.
- »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«
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* '''Apr-07 Mo'''
* '''Apr-08 We'''
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="100" align="center" | [[Image:Logo OROBOROS-DatLab.jpg|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]][[File:Blue-info.png|25px|Improve]]
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.'''
|'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?'''
- DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - »'''[[SUIT-010 |SUIT-010]]'''«
- If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]].
- »[[SUIT: Browse DL-Protocols and templates]]«
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* '''Apr-02 Mo'''
* '''Apr-02 Th'''  
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]  
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]][[File:Blue-info.png|25px|Improve]]
|'''Do you want to search the best [[SUIT]] protocol for your research questions?''' Use the SUITbrowser: »'''[[SUITbrowser]]'''«
|'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?'''
- '''Do you want to search the best [[SUIT]] protocol for your research questions?'''
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«
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|}
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== Mar 2020 ==
== Mar 2020 ==

Revision as of 10:04, 4 May 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

May 2020

  • May-11 Th
O2k-Open SupportTake care Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.

- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. - »Steady state«


  • May-04 Mo
O2k-Open SupportTake care Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels?

- See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«

Apr 2020

  • Apr-27 Mo
O2k-Open SupportTake care Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O2 regime?

- To obtain an accurate O2 background correction, you must select the marks that correspond to your range of experimental O2 levels. - » O2 Background correction - troubleshooting«

  • Apr-20 Mo
O2k-Open SupportImprove Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?

- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation. - » Pyruvate - troubleshooting«

  • Apr-14 Mo
O2k-Open SupportTake care Why are the oxygen levels different between chambers prior to my experimental run?

- After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations. - » Oxygen calibration - troubleshooting«

  • Apr-07 Mo
60px DatLabImprove Did you know that you can edit the background parameters in the 'O2 analysis template DL7.4?

- If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4. - »SUIT: Browse DL-Protocols and templates«

  • Apr-02 Mo
MitoPedia: SUITImprove Did you know that you can edit the background parameters in the 'O2 analysis template DL7.4?

- Do you want to search the best SUIT protocol for your research questions? - Use the SUITbrowser: »SUITbrowser«

Mar 2020

  • Mar-16 Mo
MitoPedia: SUIT The standardized coupling control protocol to explore the bioenergetics of living cells

- DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«


  • Mar-11 We
MitoPedia Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.

- »Blebbistatin«


  • Mar-06 Fr
MitoPedia: SUIT Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.

- »RP1« and »RP2«


  • Mar-03 Tu
MitoPedia Rapamycin - more differences or more in common with other mitObesity drugs?

- »Rapamycin«


Feb 2020

  • Feb-26 We
MitoPedia It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«


  • Feb-24 Mo
MitoPedia More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«


  • Feb-19 We
MitoPedia Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«


  • Feb-18 Tu
MitoPedia Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«


  • Feb-12 We
MitoPedia What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«


Jan 2020

  • Jan-16 Th
MitoPedia Oxygen flux

- which SI units should be used?


MitoPedia Did you ever think that units for body mass could be an issue?

- »Body mass«


  • Jan-08 We
MitoPedia Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.


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