Nagwani 2023 MiPschool Obergurgl

Nagwani Amit K (2023) Mitochondria functionality in tardigrades under the hypomagnetic field . MiPschool Obergurgl 2023.

Link: MiPschool Obergurgl 2023

Nagwani Amit K (2023)

Event: MiPschool Obergurgl 2023

Introduction: Tardigrades are considered as one of the toughest animals on Earth due to their remarkable ability to withstand extreme condition. An example of these conditions is hypomagnetic field (HMF, static magnetic field with an intensity <5 μT), which is known to influence the metabolic processes including mitochondria functioning. However, very few studies considering HMF impact were performed for organisms able to survive under extreme conditions and considered as suitable for outer space colonization. Therefore, we decided to check the impact of HMF on the tardigrade Paramacrobiotus experimentalis focusing on mitochondria functionality reflected by the mitochondrial inner membrane potential (ΔΨ) having regard to age and sex. Methods: Females and males from 3 different age classes (i.e., 30-60, 150-180 and >300 days) were extracted from laboratory culture and divided into experimental and control groups exposed to HMF and standard magnetic field (SMF), respectively, for three different durations i.e., 7 days, 15 days and 30 days. The HMF treatment was performed in a special anti-magnetic chamber whereas SMF treatment was performed in a climate chamber. TMRM staining of intact animals was used to estimate ΔΨ. Results and discussion: The calculated FITMRM index indicated HMF-related changes in Δψ dependent on age and sex. Accordingly, HMF effect was most pronounced for the oldest animals and males appeared to be more sensitive to HMF than females that correlated with the survival rate. The results provide an insight into mechanisms of HMF effect that could be useful for organization of space travels and living outside the Earth. References: 1. WeiChuan M, Ying L, RongQiao HE (2014) Hypomagnetic field, an ignorable environmental factor in space? Science China Life sciences; 57: 726-728 2. Binhi VN, Prato FS (2017). Biological effects of the hypomagnetic field: An analytical review of experiments and theories. PLOS ONE; 12 : e0179340 3. Conley CC (1970) A Review of the biological effects of very low magnetic fields. NASA. Technical Note; TN D-5902: 1–27 4. Erdmann W, Idzikowski B, Kowalski W, Kosicki J, Kaczmarek Ł (2021) Tolerance of two anhydrobiotic tardigrades Echiniscus testudo and Milnesium inceptum to hypomagnetic conditions. PeerJ; 9: e10630

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