Nagwani 2023 MiPschool Obergurgl: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=[[Image:MiPsocietyLOGO.JPG|left|100px|Mitochondrial Physiology Society|MiPsociety]] Mitochondria functionality in tardigrades under the hypomagnetic field. | |title=[[Image:MiPsocietyLOGO.JPG|left|100px|Mitochondrial Physiology Society|MiPsociety]] Mitochondria functionality in tardigrades under the hypomagnetic field. | ||
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|year=2023 | |year=2023 | ||
|event=MiPschool Obergurgl 2023 | |event=MiPschool Obergurgl 2023 | ||
|abstract='''Authors:''' [[Nagwani Amit K]], [[Kaczmarek L]], [[Kmita H]] <br> | |abstract='''Authors:''' [[Nagwani Amit K]], [[Kaczmarek L]], [[Kmita H]] <br><br> | ||
'''Introduction:''' Tardigrades are considered as one of the toughest animals on Earth due to their remarkable ability to withstand extreme condition. An example of these conditions is hypomagnetic field (HMF, static magnetic field with an intensity <5βΞΌT), which is known to influence the metabolic processes including mitochondria functioning. However, very few studies considering HMF impact were performed for organisms able to survive under extreme conditions and considered as suitable for outer space colonization. Therefore, we decided to check the impact of HMF on the tardigrade Paramacrobiotus experimentalis focusing on mitochondria functionality reflected by the mitochondrial inner membrane potential ( | '''Introduction:''' Tardigrades are considered as one of the toughest animals on Earth due to their remarkable ability to withstand extreme condition. An example of these conditions is hypomagnetic field (HMF, static magnetic field with an intensity <5βΞΌT), which is known to influence the metabolic processes including mitochondria functioning. However, very few studies considering HMF impact were performed for organisms able to survive under extreme conditions and considered as suitable for outer space colonization. Therefore, we decided to check the impact of HMF on the tardigrade ''Paramacrobiotus experimentalis'' focusing on mitochondria functionality reflected by the mitochondrial inner membrane potential (Ξ<sub>Ξ¨</sub>) having regard to age and sex. <br> | ||
'''Methods:''' Females and males from 3 different age classes (i.e., 30-60, 150-180 and >300 days) were extracted from laboratory culture and divided into experimental and control groups exposed to HMF and standard magnetic field (SMF), respectively, for three different durations i.e., 7 days, 15 days and 30 days. The HMF treatment was performed in a special anti-magnetic chamber whereas SMF treatment was performed in a climate chamber. TMRM staining of intact animals was used to estimate | '''Methods:''' Females and males from 3 different age classes (i.e., 30-60, 150-180 and >300 days) were extracted from laboratory culture and divided into experimental and control groups exposed to HMF and standard magnetic field (SMF), respectively, for three different durations i.e., 7 days, 15 days and 30 days. The HMF treatment was performed in a special anti-magnetic chamber whereas SMF treatment was performed in a climate chamber. TMRM staining of intact animals was used to estimate Ξ<sub>Ξ¨</sub>. <br> | ||
'''Results and discussion:''' The calculated FITMRM index indicated HMF-related changes in ΞΟ dependent on age and sex. Accordingly, HMF effect was most pronounced for the oldest animals and males appeared to be more sensitive to HMF than females that correlated with the survival rate. The results provide an insight into mechanisms of HMF effect that could be useful for organization of space travels and living outside the Earth. <br> | '''Results and discussion:''' The calculated FITMRM index indicated HMF-related changes in ΞΟ dependent on age and sex. Accordingly, HMF effect was most pronounced for the oldest animals and males appeared to be more sensitive to HMF than females that correlated with the survival rate. The results provide an insight into mechanisms of HMF effect that could be useful for organization of space travels and living outside the Earth. <br> | ||
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== Affiliations == | == Affiliations == | ||
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::::# Dept. of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University, PoznaΕ, Poland | ::::# Dept. of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University, PoznaΕ, Poland | ||
:::: Corresponding author: [email protected] | :::: Corresponding author: [email protected] | ||
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|organism=Other invertebrates | |||
|event=E1 | |||
}} | |||
Latest revision as of 16:08, 28 June 2023
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Nagwani 2023 MiPschool Obergurgl
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Link: MiPschool Obergurgl 2023
Nagwani Amit K (2023)
Event: MiPschool Obergurgl 2023
Authors: Nagwani Amit K, Kaczmarek L, Kmita H
Introduction: Tardigrades are considered as one of the toughest animals on Earth due to their remarkable ability to withstand extreme condition. An example of these conditions is hypomagnetic field (HMF, static magnetic field with an intensity <5βΞΌT), which is known to influence the metabolic processes including mitochondria functioning. However, very few studies considering HMF impact were performed for organisms able to survive under extreme conditions and considered as suitable for outer space colonization. Therefore, we decided to check the impact of HMF on the tardigrade Paramacrobiotus experimentalis focusing on mitochondria functionality reflected by the mitochondrial inner membrane potential (ΞΞ¨) having regard to age and sex.
Methods: Females and males from 3 different age classes (i.e., 30-60, 150-180 and >300 days) were extracted from laboratory culture and divided into experimental and control groups exposed to HMF and standard magnetic field (SMF), respectively, for three different durations i.e., 7 days, 15 days and 30 days. The HMF treatment was performed in a special anti-magnetic chamber whereas SMF treatment was performed in a climate chamber. TMRM staining of intact animals was used to estimate ΞΞ¨.
Results and discussion: The calculated FITMRM index indicated HMF-related changes in ΞΟ dependent on age and sex. Accordingly, HMF effect was most pronounced for the oldest animals and males appeared to be more sensitive to HMF than females that correlated with the survival rate. The results provide an insight into mechanisms of HMF effect that could be useful for organization of space travels and living outside the Earth.
- Mo W, Liu Y, He R. (2014) Hypomagnetic field, an ignorable environmental factor in space? https://doi.org/10.1007/s11427-014-4662-x
- Binhi VN, Prato FS (2017) Biological effects of the hypomagnetic field: An analytical review of experiments and theories. https://doi.org/10.1371/journal.pone.0179340
- Conley CC (1970) A Review of the biological effects of very low magnetic fields. NASA. Technical Note; TN D-5902: 1β27. https://ntrs.nasa.gov/citations/19700024915
- Erdmann W, Idzikowski B, Kowalski W, Kosicki J, Kaczmarek Ε (2021) Tolerance of two anhydrobiotic tardigrades Echiniscus testudo and Milnesium inceptum to hypomagnetic conditions. https://doi.org/10.7717/peerj.10630
Affiliations
- Nagwani Amit K1, Kaczmarek L2,Kmita H
- Dept. of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, PoznaΕ, Poland
- Dept. of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University, PoznaΕ, Poland
- Corresponding author: [email protected]
- Nagwani Amit K1, Kaczmarek L2,Kmita H
Labels:
Organism: Other invertebrates
Event: E1
