- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Myxothiazol is an inhibitor of CIII (inhibits also CI; G. Lenaz). Myxothiazol binds to the Qo site of CIII (very close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske Iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Myx: Myxothiazol (Myxothiazol from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7.
Caution: Very toxic.
Substance is again available from Sigma, but with the product number cited above. The old product number was M 5779.
Preparation of 1 mM stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 1 mg of myxothiazol into a small glass vial.
- 2) Add 2.05 ml ethanol.
- 3) Divide into 0.2 ml (use glass vials).
- 4) Store at -20 °C.
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe.
- Final conc. in 2 ml O2k-chamber: 0.5 µM.
