- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Inhibitor of CIII (inhibits also CI; G. Lenaz). Myxothiazol binds to the Qo site of Complex III (very close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske Iron sulfur protein [1].
Abbreviation: Myx
Reference: MiPNet09.12
Labels:
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Myx: Myxothiazol (Myxothiazol from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma M 5779, 1 mg, store at 4 °C: FW = 487.7
Caution: Very toxic. Chemical is currently not available 2010-11-22. Alternatively use Antimycin A as a Complex III inhibitor.
Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 1 mg of Myxothiazol into a small glass vial
- 2) add 2.05 ml Ethanol
- 3) divide into 0.2 ml (use glass vials)
- 4) store at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM
