Difference between revisions of "Myxothiazol"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=Myx | |abbr=Myx | ||
|description=Inhibitor of [CIII] (inhibits also [CI]; G. Lenaz). | |description=Inhibitor of [[Complex III|CIII]] (inhibits also [[Complex I|CI]]; G. Lenaz). | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
Revision as of 12:54, 22 November 2010
- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Inhibitor of CIII (inhibits also CI; G. Lenaz).
Abbreviation: Myx
Reference: MiPNet09.12
Labels:
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Myx: Myxothiazol (Myxothiazol from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma M 5779, 1 mg, store at 4 °C): FW = 487.7)
Caution: Very toxic. Chemical is currently not available 2010-11-22. Alternatively use Antimycin A as a Complex III inhibitor.
Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 1 mg of Myxothiazol directly into a 2 ml glass vial
- 2) add 2.05 ml Ethanol
- 3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 4) store in glass vials (recommendation) at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM
