Difference between revisions of "Myxothiazol"
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== Application in [[HRR]] == | |||
'''Myx: Myxothiazol''' (Myxothiazol from ''Myxobacterium myxococcus fulvus'', ~95%, HPLC), C<sub>25</sub>H<sub>33</sub>N<sub>3</sub>O<sub>3</sub>S<sub>2</sub>); Sigma M 5779, 1 mg, store at 4 °C): FW = 487.7) | |||
<span style="color:#8B008B"> '''Caution:''' Very toxic. Chemical is currently not available 2010-11-22. Alternatively use [[Antimycin A]] as a Complex III inhibitor.</span> | |||
'''Preparation of 1 mM stock solution''' (dissolved in 99.9% Ethanol): | |||
::1) weigh 491.2 mg of ADP (1 mmol ADP) | |||
::2) add 1.3 ml H<sub>2</sub>O !White precipitate will occur, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature! | |||
::3) neutralize with 5 N KOH (approx. 465 µl) !ADP will dissolve after addition of KOH! (this is '''Solution A''') | |||
::4) check pH and adjust to 7 if necessary | |||
::5) adjust final volume to 2 ml and divide into 0.2 ml portions | |||
::6) store frozen at -80 °C | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | |||
::* Titration volume: 1 µl using a 10 µl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 0.5 µM | |||
Revision as of 12:52, 22 November 2010
- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Inhibitor of [CIII] (inhibits also [CI]; G. Lenaz).
Abbreviation: Myx
Reference: MiPNet09.12
Labels:
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Myx: Myxothiazol (Myxothiazol from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma M 5779, 1 mg, store at 4 °C): FW = 487.7)
Caution: Very toxic. Chemical is currently not available 2010-11-22. Alternatively use Antimycin A as a Complex III inhibitor.
Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 491.2 mg of ADP (1 mmol ADP)
- 2) add 1.3 ml H2O !White precipitate will occur, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature!
- 3) neutralize with 5 N KOH (approx. 465 µl) !ADP will dissolve after addition of KOH! (this is Solution A)
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 2 ml and divide into 0.2 ml portions
- 6) store frozen at -80 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM
