Difference between revisions of "Myxothiazol"
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|description='''Myxothiazol''' is an inhibitor of [[Complex III|CIII]] (inhibits also [[Complex I|CI]]; G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein. | |description='''Myxothiazol''' is an inhibitor of [[Complex III|CIII]] (inhibits also [[Complex I|CI]]; G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
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{{MitoPedia methods}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 09:38, 27 March 2015
- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Myxothiazol is an inhibitor of CIII (inhibits also CI; G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
Caution: Very toxic.
Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 1 mg of myxothiazol into a small glass vial.
- 2) Add 2.05 ml ethanol.
- 3) Divide into 0.2 ml (use glass vials).
- 4) Store at -20 °C.
Oxygraph-2k manual titrations MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 0.5 µM.
