Difference between revisions of "Myxothiazol"
From Bioblast
| Line 13: | Line 13: | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Myx: Myxothiazol''' ( | '''Myx: Myxothiazol''' (from ''Myxobacterium myxococcus fulvus'', ~95%, HPLC), C<sub>25</sub>H<sub>33</sub>N<sub>3</sub>O<sub>3</sub>S<sub>2</sub>); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779. | ||
<span style="color:#8B008B"> '''Caution:''' Very toxic. | <span style="color:#8B008B"> '''Caution:''' Very toxic.</span> | ||
'''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol): | |||
'''Preparation of 1 mM stock solution''' (dissolved in 99.9% ethanol): | |||
::1) Weigh 1 mg of myxothiazol into a small glass vial. | ::1) Weigh 1 mg of myxothiazol into a small glass vial. | ||
| Line 28: | Line 26: | ||
'''Oxygraph-2k | '''Oxygraph-2k manual titrations''' [[MiPNet09.12]] | ||
::* Titration volume: 1 µl using a 10 µl syringe. | ::* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber). | ||
::* Final | ::* Final concentration: 0.5 µM. | ||
== Compare == | == Compare == | ||
* [[Antimycin A]] | * [[Antimycin A]] | ||
Revision as of 15:27, 2 May 2014
- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Myxothiazol is an inhibitor of CIII (inhibits also CI; G. Lenaz). Myxothiazol binds to the Qo site of CIII (very close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske Iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
Caution: Very toxic.
Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 1 mg of myxothiazol into a small glass vial.
- 2) Add 2.05 ml ethanol.
- 3) Divide into 0.2 ml (use glass vials).
- 4) Store at -20 °C.
Oxygraph-2k manual titrations MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 0.5 µM.
