Difference between revisions of "Myxothiazol"

Revision as of 05:23, 1 February 2014

high-resolution terminology - matching measurements at high-resolution

Myxothiazol

Description

Myxothiazol is an inhibitor of CIII (inhibits also CI; G. Lenaz). Myxothiazol binds to the Qo site of CIII (very close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske Iron sulfur protein.

Abbreviation: Myx

Reference: MiPNet09.12

MitoPedia topics: Inhibitor

Application in HRR

Myx: Myxothiazol (Myxothiazol from Myxobacterium myxococcus fulvus, ~95%, HPLC), C₂₅H₃₃N₃O₃S₂); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7.

Caution: Very toxic.

Substance is again available from Sigma, but with the product number cited above. The old product number was M 5779.

Preparation of 1 mM stock solution (dissolved in 99.9% ethanol):

1) Weigh 1 mg of myxothiazol into a small glass vial.

2) Add 2.05 ml ethanol.

3) Divide into 0.2 ml (use glass vials).

4) Store at -20 °C.

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 1 µl using a 10 µl syringe.
Final conc. in 2 ml O2k-chamber: 0.5 µM.

Compare

Antimycin A

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Myx
-|description='''Myxothiazol''' is an inhibitor of [[Complex III|CIII]] (inhibits also [[Complex I|CI]]; G. Lenaz). Myxothiazol binds to the Qo site of CIII (very close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske Iron sulfur protein [http://en.wikipedia.org/wiki/Coenzyme_Q_-_cytochrome_c_reductase#Inhibitors_of_complex_III].
+|description='''Myxothiazol''' is an inhibitor of [[Complex III|CIII]] (inhibits also [[Complex I|CI]]; G. Lenaz). Myxothiazol binds to the Qo site of CIII (very close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske Iron sulfur protein.
 |info=[[MiPNet09.12]]
 |type=Inhibitor ETS
+}}
+{{MitoPedia methods|type=Inhibitor ETS
 }}
 {{MitoPedia topics
@@ Line 9: / Line 11: @@
 |type=Inhibitor ETS
 }}
-{{Labeling
-|kinetics=Inhibitor; Uncoupler
-|instruments=Chemicals; Media
-|type=Inhibitor ETS
-}}
 == Application in [[HRR]] ==
-'''Myx: Myxothiazol''' (Myxothiazol from ''Myxobacterium myxococcus fulvus'', ~95%, HPLC), C<sub>25</sub>H<sub>33</sub>N<sub>3</sub>O<sub>3</sub>S<sub>2</sub>); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7
+'''Myx: Myxothiazol''' (Myxothiazol from ''Myxobacterium myxococcus fulvus'', ~95%, HPLC), C<sub>25</sub>H<sub>33</sub>N<sub>3</sub>O<sub>3</sub>S<sub>2</sub>); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7.
 <span style="color:#8B008B"> '''Caution:''' Very toxic.
-Substance is again available from Sigma, but with the product number cited above. The old product number was M 5779. Alternatively use [[Antimycin A]] as a Complex III inhibitor.</span>
+Substance is again available from Sigma, but with the product number cited above. The old product number was M 5779.</span>
-'''Preparation of 1 mM stock solution''' (dissolved in 99.9% Ethanol):
+'''Preparation of 1 mM stock solution''' (dissolved in 99.9% ethanol):
-::1) weigh 1 mg of Myxothiazol into a small glass vial
+::1) Weigh 1 mg of myxothiazol into a small glass vial.
-::2) add 2.05 ml Ethanol
+::2) Add 2.05 ml ethanol.
-::3) divide into 0.2 ml (use glass vials)
+::3) Divide into 0.2 ml (use glass vials).
-::4) store at -20 °C
+::4) Store at -20 °C.
 '''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
-::* Titration volume: 1 µl using a 10 µl syringe
+::* Titration volume: 1 µl using a 10 µl syringe.
-::* Final conc. in 2 ml O2k-chamber: 0.5 µM
+::* Final conc. in 2 ml O2k-chamber: 0.5 µM.
+== Compare ==
+* [[Antimycin A]]