Difference between revisions of "Myxothiazol"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=Myx | |abbr=Myx | ||
|description='''Myxothiazol''' Myx is an inhibitor of [[Complex III]] (CIII). CIII also inhibits [[Complex I|CI]] | |description='''Myxothiazol''' Myx is an inhibitor of [[Complex III]] (CIII). CIII also inhibits [[Complex I|CI]]. Myxothiazol binds to the Q<sub>o</sub> site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH<sub>2</sub> to the Rieske iron sulfur protein. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
}} | }} | ||
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|complete name=from ''Myxobacterium myxococcus fulvus'' | |complete name=from ''Myxobacterium myxococcus fulvus'' | ||
|chem formula=C<sub>25</sub>H<sub>33</sub>N<sub>3</sub>O<sub>3</sub>S<sub>2</sub> | |chem formula=C<sub>25</sub>H<sub>33</sub>N<sub>3</sub>O<sub>3</sub>S<sub>2</sub> | ||
|molar mass=487.7 | |molar mass=487.7 | ||
|vendor=Sigma-Aldrich | |vendor=Sigma-Aldrich | ||
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:::: Available again from Sigma with this new product number. The old product number was M 5779. | :::: Available again from Sigma with this new product number. The old product number was M 5779. | ||
<!--::::<span style="color:#8B008B"> '''Caution:''' Very toxic.</span>--> | <!--::::<span style="color:#8B008B"> '''Caution:''' Very toxic.</span>--> | ||
::::'''Preparation of 1 mM storage and stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''': | ::::'''Preparation of 1 mM storage and stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''': | ||
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::::'''Preparation of 0. | ::::'''Preparation of 0.25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-chamber''': | ||
::::# Take 100 µL of the 1 mM stock solution. | ::::# Take 100 µL of the 1 mM stock solution. | ||
::::# Add 300 µL | ::::# Add 300 µL EtOH. | ||
:::»'''O2k manual titrations''' [[MiPNet09.12]] | :::»'''O2k manual titrations''' [[MiPNet09.12]] | ||
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== References == | |||
::::* Von Jagow G, Engel WD(1981) Complete inhibition of electron transfer from ubiquinol to cytochrome b by the combined action of antimycin and myxothiazol. FEBS Lett 136:19-24. https://doi.org/10.1016/0014-5793(81)81206-9 | |||
::::* Von Jagow G, Ljungdahl PO, Graf P, Ohnishi T, Trumpower BL (1984) An inhibitor of mitochondrial respiration which binds to cytochrome b and displaces quinone from the iron-sulfur protein of the cytochrome bc1 complex. J Biol Chem 259:6318–26. https://doi.org/10.1016/S0021-9258(20)82143-7 | |||
{{MitoPedia O2k and high-resolution respirometry}} | {{MitoPedia O2k and high-resolution respirometry}} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
Revision as of 18:12, 9 April 2022
- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Myxothiazol Myx is an inhibitor of Complex III (CIII). CIII also inhibits CI. Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
Application in HRR
- Myx: Myxothiazol (from Myxobacterium myxococcus fulvus; C25H33N3O3S2), Sigma-Aldrich: T5580, store at 4 °C, CAS: 76706-55-3, M = 487.7 g·mol-1
- Hazard statements: H300; fatal if swallowed
- Myx: Myxothiazol (from Myxobacterium myxococcus fulvus; C25H33N3O3S2), Sigma-Aldrich: T5580, store at 4 °C, CAS: 76706-55-3, M = 487.7 g·mol-1
- Available again from Sigma with this new product number. The old product number was M 5779.
- Preparation of 1 mM storage and stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- Weigh 1 mg of myxothiazol into a small glass vial.
- Add 2.05 mL ethanol.
- Divide into 0.2 mL (use glass vials).
- Store at -20 °C.
- Preparation of 1 mM storage and stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- »O2k manual titrations MiPNet09.12
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 0.5 µM.
- »O2k manual titrations MiPNet09.12
- Preparation of 0.25 mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- Take 100 µL of the 1 mM stock solution.
- Add 300 µL EtOH.
- Preparation of 0.25 mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- »O2k manual titrations MiPNet09.12
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 0.5 µM.
- »O2k manual titrations MiPNet09.12
Compare
- Antimycin A is less expensive than Myx but induces higher ROS production.
References
- Von Jagow G, Engel WD(1981) Complete inhibition of electron transfer from ubiquinol to cytochrome b by the combined action of antimycin and myxothiazol. FEBS Lett 136:19-24. https://doi.org/10.1016/0014-5793(81)81206-9
- Von Jagow G, Ljungdahl PO, Graf P, Ohnishi T, Trumpower BL (1984) An inhibitor of mitochondrial respiration which binds to cytochrome b and displaces quinone from the iron-sulfur protein of the cytochrome bc1 complex. J Biol Chem 259:6318–26. https://doi.org/10.1016/S0021-9258(20)82143-7
MitoPedia topics:
Inhibitor
