Difference between revisions of "Myxothiazol"

Revision as of 11:50, 28 October 2020

high-resolution terminology - matching measurements at high-resolution

Myxothiazol

Description

Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome b_L) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.

Abbreviation: Myx

Reference: MiPNet09.12

Application in HRR

Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C₂₅H₃₃N₃O₃S₂); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.

Caution: Very toxic.

Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol) for 2-mL O2k-chamber:

Weigh 1 mg of myxothiazol into a small glass vial.
Add 2.05 mL ethanol.
Divide into 0.2 mL (use glass vials).
Store at -20 °C.

»O2k manual titrations MiPNet09.12

Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
Final concentration: 0.5 µM.

Preparation of 0.25mM stock solution (dissolved in 99.9% ethanol) for 0.5-mL O2k-chamber:

Take 100 µL of the 1 mM stock solution.
Add 300 µL ethanol.

»O2k manual titrations MiPNet09.12

Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
Final concentration: 0.5 µM.

Compare

Antimycin A is less expensive than Myx but induces higher ROS production.

MitoPedia topics: Inhibitor

@@ Line 12: / Line 12: @@
 ::::<span style="color:#8B008B"> '''Caution:''' Very toxic.</span>
-::::'''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol):
+::::'''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol) for '''2-mL O2k-chamber''':
 ::::# Weigh 1 mg of myxothiazol into a small glass vial.
 ::::# Add 2.05 mL ethanol.
@@ Line 18: / Line 18: @@
 ::::# Store at -20 °C.
+:::»'''O2k manual titrations'''  [[MiPNet09.12]]
+::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
+::::* Final concentration: 0.5 µM.
+::::'''Preparation of 0.25mM stock solution''' (dissolved in 99.9% ethanol) for '''0.5-mL O2k-chamber''':
+::::# Take 100 µL of the 1 mM stock solution.
+::::# Add 300 µL ethanol.
 :::»'''O2k manual titrations'''  [[MiPNet09.12]]
-::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-chamber).
+::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
 ::::* Final concentration: 0.5 µM.
 == Compare ==