Difference between revisions of "Myxothiazol"
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|description='''Myxothiazol''', Myx, is an inhibitor of [[Complex III]] (CIII). CIII also inhibits [[Complex I|CI]] (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein. | |description='''Myxothiazol''', Myx, is an inhibitor of [[Complex III]] (CIII). CIII also inhibits [[Complex I|CI]] (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
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== Compare == | == Compare == | ||
* [[Antimycin A]] is less expensive than Myx but induces higher ROS production. | * [[Antimycin A]] is less expensive than Myx but induces higher ROS production. | ||
}} | |||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
Revision as of 16:28, 8 September 2020
- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
Application in HRR
Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
Caution: Very toxic.
Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol):
- Weigh 1 mg of myxothiazol into a small glass vial.
- Add 2.05 mL ethanol.
- Divide into 0.2 mL (use glass vials).
- Store at -20 °C.
O2k manual titrations MiPNet09.12
- Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-chamber).
- Final concentration: 0.5 µM.
Compare
- Antimycin A is less expensive than Myx but induces higher ROS production.
MitoPedia topics: Inhibitor
