Difference between revisions of "Mozo 2006 Biochem J"
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role of its close homologues, UCP2 and UCP3, is unclear.Mounting | role of its close homologues, UCP2 and UCP3, is unclear.Mounting | ||
evidence associates them with the control of mitochondrial | evidence associates them with the control of mitochondrial | ||
superoxide production. | superoxide production. Using CHO(Chinese-hamster ovary) cells | ||
stably expressing UCP3 or UCP1, we found no evidence for respiration | stably expressing UCP3 or UCP1, we found no evidence for respiration | ||
uncoupling. The explanation lies in the absence of an | uncoupling. The explanation lies in the absence of an | ||
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{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|organism= | |organism=Mammals | ||
|preparations=Intact Cell; Cultured; Primary | |preparations=Intact Cell; Cultured; Primary | ||
|kinetics=ADP; Pi, Inhibitor; Uncoupler, Temperature | |kinetics=ADP; Pi, Inhibitor; Uncoupler, Temperature | ||
|topics=Substrate; Glucose; TCA Cycle | |topics=Substrate; Glucose; TCA Cycle | ||
}} | }} | ||
Revision as of 18:13, 29 January 2013
| Mozo J, Ferry G, Studeny A, Pecqueur C, Rodriguez M, Boutin JA, Bouillaud F (2006) Expression of UCP3 in CHO cells does not cause uncoupling but controls mitochondrial activity in the presence of glucose. Biochem J 393: 431-439. |
Mozo J, Ferry G, Studeny A, Pecqueur C, Rodriguez M, Boutin JA, Bouillaud F (2006) Biochem J
Abstract: The proton-transport activity of UCP1 (uncoupling protein 1) triggers mitochondrial uncoupling and thermogenesis. The exact role of its close homologues, UCP2 and UCP3, is unclear.Mounting evidence associates them with the control of mitochondrial superoxide production. Using CHO(Chinese-hamster ovary) cells stably expressing UCP3 or UCP1, we found no evidence for respiration uncoupling. The explanation lies in the absence of an appropriate activator of UCP protonophoric function. Accordingly, the addition of retinoic acid uncouples the respiration of the UCP1-expressing clone, but not that of the UCP3-expressing ones. In a glucose-containing medium, the extent of the hyperpolarization of mitochondria by oligomycin was close to 22 mV in the five UCP3-expressing clones, contrasting with the variable values observed with the 15 controls. Our observations suggest that, when glycolysis and mitochondria generate ATP, and in the absence of appropriate activators of proton transport, UCPs do not transport protons (uncoupling), but rather other ions of physiological relevance that control mitochondrial activity. A model is proposed using the known passive transport of pyruvate by UCP1. • Keywords: Glycolysis, Membrane potential, Mitochondria, Pyruvate, Reactive oxygen species, Uncoupling protein (UCP)
• O2k-Network Lab: FR_Paris_Bouillaud F
Labels:
Organism: Mammals"Mammals" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
