MitoOx2: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=MitoOx2 | |abbr=MitoOx2 | ||
|description='''Mitochondrial respiration medium, MitoOx2''', developed for oxygraph incubations of [[mitochondrial preparations]] | |description='''Mitochondrial respiration medium, MitoOx2''', developed for oxygraph incubations of [[mitochondrial preparations]] to measure the H<sub>2</sub>O<sub>2</sub> production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H<sub>2</sub>O<sub>2</sub> present) for Amplex UltraRed(R) than e.g. [[MiR05|MiR05]]. | ||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia concepts|type=Chemicals | ||
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|type=Chemicals | {{MitoPedia methods|type=Chemicals | ||
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{{MitoPedia O2k and high-resolution respirometry|type=Chemicals | |||
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{{MitoPedia topics | {{MitoPedia topics | ||
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|type=Chemicals | |type=Chemicals | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''MitoOx2: Mitochondrial Respiration Medium for measuring the H<sub>2</sub>O<sub>2</sub> production''' ย | '''MitoOx2: Mitochondrial Respiration Medium for measuring the H<sub>2</sub>O<sub>2</sub> production''' ย | ||
::Oxygen solubility factor of | ::Oxygen solubility factor of MitoOx2 at 30ยฐC and 37ยฐC = 0.95 | ||
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{| class="wikitable" | {| class="wikitable" | ||
|- | |- | ||
! Compound !! Final conc. !! | ! Compound !! Final conc. !! M [gยทmol<sup>-1</sup>] !! Company, product code and storage | ||
|- | |- | ||
| KCl || 120 mM || 74.55 || align="right" |Sigma 31248, store at RT | | KCl || 120 mM || 74.55 || align="right" |Sigma 31248, store at RT | ||
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| MgCl<sub>2</sub>|| 2.86 mM || 203.30 || align="right" | Scharlau MA0036, store at RT | | MgCl<sub>2</sub>|| 2.86 mM || 203.30 || align="right" | Scharlau MA0036, store at RT | ||
|- | |- | ||
| EGTA free acid || | | EGTA free acid || 0.2 mM || 380.35 || align="right" | Sigma E-4378, store at RT | ||
|- | |- | ||
| BSA essentially fatty acid free || 0.025% || - || align="right" | Sigma A-6003, store at 2 - 8 ยฐC | | BSA essentially fatty acid free || 0.025% || - || align="right" | Sigma A-6003, store at 2 - 8 ยฐC | ||
|} | |} | ||
adjust pH to 7.0 (room temperature) with KOH |
Latest revision as of 13:08, 12 February 2021
Description
Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations to measure the H2O2 production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H2O2 present) for Amplex UltraRed(R) than e.g. MiR05.
Abbreviation: MitoOx2
MitoPedia topics: Media for respirometry
Application in HRR
MitoOx2: Mitochondrial Respiration Medium for measuring the H2O2 production
- Oxygen solubility factor of MitoOx2 at 30ยฐC and 37ยฐC = 0.95
Chemicals for MitoOx2
Compound | Final conc. | M [gยทmol-1] | Company, product code and storage |
---|---|---|---|
KCl | 120 mM | 74.55 | Sigma 31248, store at RT |
HEPES free acid | 20 mM | 238.31 | Sigma H-7523 store at RT |
KH2PO4 | 10 mM | 136.09 | Sigma P-5655, store at RT |
MgCl2 | 2.86 mM | 203.30 | Scharlau MA0036, store at RT |
EGTA free acid | 0.2 mM | 380.35 | Sigma E-4378, store at RT |
BSA essentially fatty acid free | 0.025% | - | Sigma A-6003, store at 2 - 8 ยฐC |
adjust pH to 7.0 (room temperature) with KOH