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| <big><big><big>'''WG2''' | | <big><big><big>'''WG2 Project application''' |
| :::: '''MITOEAGLE data repository in muscle and other tissues''' | | :::: '''MitoEAGLE data repository in skeletal and cardiac muscle''' |
| </big></big></big> | | </big></big></big> |
| | ::::» [[WG2 MitoEAGLE data: muscle |WG2 Action]] |
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| | [[File:MITOEAGLE Working groups.jpg|right|400px|MitoEAGLE Working Groups]] |
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| [[File:MITOEAGLE Working groups.jpg|right|400px|MITOEAGLE Working Groups]]
| | = WG2 Project application = |
| | | == Description == |
| = Project application = | |
| == WG2 Description == | |
| :::: During the past 10 years, a dramatically increasing number of studies report respirometric data on human muscle tissue (e.g. Christiansen 2015 Am J Physiol). Muscle mitochondrial function is particularly studied in genetically engineered mouse models of human diseases (e.g. Jelenik 2014 Diabetes). However, despite the many published data, lack of consistency often precludes quantitative comparison among data sets with permeabilized or homogenized muscle fibres or isolated mitochondria, normalized to either tissue wet weight, dry weight, protein content, citrate synthase activity or other mitochondrial markers. In the absence of validated conversion factors, these data remain largely unconnected and can only be qualitatively linked. | | :::: During the past 10 years, a dramatically increasing number of studies report respirometric data on human muscle tissue (e.g. Christiansen 2015 Am J Physiol). Muscle mitochondrial function is particularly studied in genetically engineered mouse models of human diseases (e.g. Jelenik 2014 Diabetes). However, despite the many published data, lack of consistency often precludes quantitative comparison among data sets with permeabilized or homogenized muscle fibres or isolated mitochondria, normalized to either tissue wet weight, dry weight, protein content, citrate synthase activity or other mitochondrial markers. In the absence of validated conversion factors, these data remain largely unconnected and can only be qualitatively linked. |
| :::: The aim of WG2 will be to provide access to complete data sets for post-study statistical analysis and use harmonization tools (WG1) for standardized documentation, to initiate a data repository on muscle tissue from humans and model organisms in health and disease. Data sets shall be obtained from already published and new studies, prompting participating labs to report experimental details beyond those already published as far as possible (using tools such as PubMed Commons). As a result, specific protocols will be summarized for handling muscle tissue. Depending on specific interests, WG2 may decide to extend their focus to other tissue types. | | :::: The aim of WG2 will be to provide access to complete data sets for post-study statistical analysis and use harmonization tools (WG1) for standardized documentation, to initiate a data repository on muscle tissue from humans and model organisms in health and disease. Data sets shall be obtained from already published and new studies, prompting participating labs to report experimental details beyond those already published as far as possible (using tools such as PubMed Commons). As a result, specific protocols will be summarized for handling muscle tissue. Depending on specific interests, WG2 may decide to extend their focus to other tissue types. |
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| == WG2 Management == | | == Management == |
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| === Tasks === | | === Tasks === |
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| === Deliverables === | | === Deliverables === |
| :::# A MITOEAGLE database on muscle tissue from humans and model organisms. | | :::# A MitoEAGLE database on muscle tissue from humans and model organisms. |
| :::# A set of guidelines for future studies on mitochondrial bioenergetics in muscle tissue. | | :::# A set of guidelines for future studies on mitochondrial bioenergetics in muscle tissue. |
| :::# Draft of review manuscript. | | :::# Draft of review manuscript. |
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| = WG2 Participants and task groups =
| | {{MITOEAGLE banner}} |
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| == WG2 Participants ==
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| {{#ask:[[MitoPedia topic::EAGLE]] [[In country::+]] [[Has COST WG2::+]] | |
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| |?In country=Country
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| |?In city=City
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| |?=Contact
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| |sort=In country
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| |limit=5000
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| == WG2 Task groups ==
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| :: ''TG leaders: suggestions discussed at the [[MitoFit Science Camp 2016 Kuehtai AT]].''
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| :: ''TG participants: listed as a summary from the feedback received.''
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| === TG2.1 Skeletal ===
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| :: TG leaders: [[Garcia-Roves Pablo M]] / [[Votion Dominique-Marie]] / [[Coen Paul M]]
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| :: TG participants: [[Chabi Beatrice]], [[Garcia-Roves Pablo M]], [[Rustan Arild]], [[Schlattner Uwe]], [[Wuest Rob C]]
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| === TG2.2 Cardiac ===
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| :: TG leaders: [[Larsen Terje S]] / [[Makrecka-Kuka Marina]]
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| :: TG participants: [[Muntean Danina M]], [[Schlattner Uwe]], [[Wuest Rob C]]
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| == WG2 Comments and discussion ==
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| ::::# ''Skeletal muscle is the main tissue we are studying in the lab.'' - '''[[Chabi B|Beatrice Chabi]]''' (2016).
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| ::::# ''For the kind of work group, our expertise is certainly most on muscle tissue (skeletal and heart), although we also worked on all kinds of permeabilized cells (primary cells and cell lines). For all these, we have extended data sets, mainly rat, but also human, from diverse experimental setups (nutritional regimen, exercise, drugs ...).'' - '''[[Schlattner U|Uwe Schlattner]]''' (2016).
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| ::::# ''In my working group we are mainly interested in muscle, especially focusing on aging and sarcopenia (using animal models). I would be happy if new cooperations could be formed through the COST Action. I could imagine working on a joint review article. Next to examining muscle from mouse models, we are studying biopsies from human heart muscle and liver, but there is no published data on these studies yet.'' - '''[[Klaus S| Susanne Klaus]]''' (2016). ''Translated by Sandra Fleischmann''
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| ::::# ''I am working in the field of mitochondrial biochemistry since many years. In detail my coworkers and I are carrying out studies on the regulation of ATP synthase and respiratory complexes in swine heart mitochondria as biological model of mammalian mitochondria. Some comparative aspects of mitochondrial bioenergetics and ATP synthase responses are also investigated in bivalve mollusk mitochondria.'' - '''[[Pagliarani A|Alessandra Pagliarani]]''' (2016).
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| ::::# '' We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest.'' - '''[[Makrecka-Kuka M| Marina Makrecka-Kuka]], [[Liepinsh E|Edgars Liepinsh]]''' (2016).
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| ::::# '' My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this!'' - '''[[Wuest RC| Rob C Wuest]]''' (2016).
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| ::::# ''We mostly work with cultured human skeletal muscle cells, from different donor groups such as lean, obese, type 2 diabetics, as well as muscle cells from athletes. We are interested in collaboration with our cells and measurements on energy metabolism and mitochondral function.'' - '''[[Rustan A| Arild Rustan]]''' (2016).
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| ::::# ''Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
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| ::::## ''Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
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| ::::## ''Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
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| ::::## ''Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - '''[[Watala C| Cezary Watala]]''' (2016).
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| ::::# ''I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action.'' - '''[[Palmeira C |Carlos Palmeira]]''' (2016).
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| ::::# ''As you know, I have received the MC nomination from our CNC in Bucharest (Ms Elena Dinu) for my colleague, Rodica Lighezan and myself. My expertise is most on muscle tissue (in particular, heart) and also, liver - so I will go for WG2 if this is fine with you. However, Both my colleague and I are mostly interested in performing respirometry in blood cells (and this is what we will start to try in the near future); thus, you decide whether we can fit both of us in WG4, or only her. Also, in order to extend the Network in other Romanian cities with powerful state medical universities, Cluj-Napoca (as you know) and Tg Mures - 2 young men, Bela Kiss and Ovidiu Cotoi have been proposed as substitute members (for gender balance too). In particular, we want to jointly ”explore” the exciting fields of mitochondrial ”hidden toxicity” and dynamics, respectively since the former colleague is a toxicologist and the latter a pathologist. I wish you good luck and I am indeed happy and proud to be part of this growing network.'' - '''[[Muntean DM|Danina M Muntean]]''' (2016).
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| ::::# ''I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining).'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''' (2016).
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| ::::# ''Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project.'' - '''[[Coen PM|Paul M Coen]]''' (2016).
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| ::::# ''In particular, my team can participate with studies about the mitochondrial function in relation to the supramolecular organization of the respiratory chain where the dynamic presence of the respiratory supercomplexes is a way to shift between the main metabolic fluxes of NAD- and FAD-dependent substrates in different metabolic conditions of the cell. Besides my basic studies on the enzyme interactions with the Coenzyme Q pool, I recently joined an Italian consortium of University laboratories whose target is to shed light on the molecular mechanisms underpinning muscle weakness and reduced muscle mass (sarcopenia) in ageing and attenuated response to exercise training stimuli in the elderly; both animal models and human subjects will be analyzed.'' - '''[[Genova ML| Maria Luisa Genova]]''' (2016).
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| == Projects in progress ==
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| ::::» [[MiPMap]]
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| === Next steps ===
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| ::::» [[MITOEAGLE data repository in muscle tissues]]
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