MiPNet17.04 CitrateSynthase
| Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr Physiol Network 08.14. |
Kuznetsov AV, Lassnig B, Gnaiger E (
Abstract: Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al., 1970; Willimas et al., 1986; Hood et al., 1989), although this role of CS has been questioned in developmental (Drahota et al., 2004) and age-related studies (Marin-Garcia et al., 1998). Proliferation of mitochondria in pathological states is normally associated with an increase in citrate synthase activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein. Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications (Kuznetsov et al., 2002; Renner et al., 2003; Hütter el al., 2004). • Keywords: Enzymes
• O2k-Network Lab: AT_Innsbruck_OROBOROS
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Preparation: Enzyme
Regulation: Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Enzymes
