Least squares method: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 6: | Line 6: | ||
|mitopedia method=Fluorometry, Spectrophotometry | |mitopedia method=Fluorometry, Spectrophotometry | ||
}} | }} | ||
{{MitoPedia | {{MitoPedia O2k and high-resolution respirometry}} | ||
{{MitoPedia topics}} | {{MitoPedia topics}} |
Latest revision as of 14:31, 8 February 2016
Description
This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spectrum of known concentration using a least squares method to match the measured spectrum with the reference spectrum. The technique results in improved accuracy compared with the use of only a few characteristic wavelengths.
MitoPedia methods: Fluorometry, Spectrophotometry