Kuznetsov 2008 Nat Protoc: Difference between revisions
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|title=Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat. Protoc. 3: 965-976. | |title=Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat. Protoc. 3: 965-976. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/18536644 PMID:18536644] | |||
|authors=Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS | |authors=Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS | ||
|year=2008 | |year=2008 | ||
|journal=Nat. Protoc. | |journal=Nat. Protoc. | ||
|abstract=Analysis of mitochondrial function is central to the study of intracellular energy metabolism, mechanisms of cell death and pathophysiology of a variety of human diseases, including myopathies, neurodegenerative diseases and cancer. However, important properties of mitochondria differ in vivo and in vitro. Here, we describe a protocol for the analysis of functional mitochondria in situ, without the isolation of organelles, in selectively permeabilized cells or muscle fibers using digitonin or saponin. A specially designed substrate/inhibitor titration approach allows the step-by-step analysis of several mitochondrial complexes. This protocol allows the detailed characterization of functional mitochondria in their normal intracellular position and assembly, preserving essential interactions with other organelles. As only a small amount of tissue is required for analysis, the protocol can be used in diagnostic settings in clinical studies. The permeabilization procedure and specific titration analysis can be completed in 2 h. | |||
|mipnetlab=EE_Tallinn_SaksV, FR_Grenoble_SaksV | |mipnetlab=EE_Tallinn_SaksV, FR_Grenoble_SaksV | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|preparations=Permeabilized Cell or Tissue; Homogenate | |||
}} | }} | ||
==Corrections required== | ==Corrections required== |
Revision as of 16:26, 30 June 2011
Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat. Protoc. 3: 965-976. |
Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Nat. Protoc.
Abstract: Analysis of mitochondrial function is central to the study of intracellular energy metabolism, mechanisms of cell death and pathophysiology of a variety of human diseases, including myopathies, neurodegenerative diseases and cancer. However, important properties of mitochondria differ in vivo and in vitro. Here, we describe a protocol for the analysis of functional mitochondria in situ, without the isolation of organelles, in selectively permeabilized cells or muscle fibers using digitonin or saponin. A specially designed substrate/inhibitor titration approach allows the step-by-step analysis of several mitochondrial complexes. This protocol allows the detailed characterization of functional mitochondria in their normal intracellular position and assembly, preserving essential interactions with other organelles. As only a small amount of tissue is required for analysis, the protocol can be used in diagnostic settings in clinical studies. The permeabilization procedure and specific titration analysis can be completed in 2 h.
โข O2k-Network Lab: EE_Tallinn_SaksV, FR_Grenoble_SaksV
Labels:
Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
Corrections required
- Table 1: Respiration of rat liver homogenate, 30 ยฐC is actually mechanically permeabilized pig liver measured at 37 ยฐC (Kuznetsov_2002_AnalytBiochem).
The protocol for permeabilized fibres lacks consideration on oxygen limitation encountered when incubations are performed at or below air saturation (Kuznetsov_1998_BTK; Gnaiger_2003_AdvExpMedBiol). Protocols are restricted to simple substrate supply (separate CI- or CII-electron entry into the ETS), whereas full OXPHOS capacity can be obtained only with physiological CI+II substrate combinations (Pesta_2011_Protocols).