Hoffman 2007 Am J Physiol Heart Circ Physiol: Difference between revisions
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|enzymes=Complex II;succinate dehydrogenase, Complex III | |enzymes=Complex II;succinate dehydrogenase, Complex III | ||
|injuries= | |injuries=Oxidative stress;RONS | ||
|topics=O2, Redox state | |topics=O2, Redox state | ||
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* For a critical review on artefacts in oxygen kinetics in the paper by Hoffman et al (2007), see [[Scandurra_2010_Adv Exp Med Biol]]. | * For a critical review on artefacts in oxygen kinetics in the paper by Hoffman et al (2007), see [[Scandurra_2010_Adv Exp Med Biol]]. |
Revision as of 13:58, 16 June 2015
Hoffman DL, Salter JD, Brookes PS (2007) Response of mitochondrial reactive oxygen species generation to steady-state oxygen tension: implications for hypoxic cell signaling. Am J Physiol Heart Circ Physiol 292:H101-8. |
Hoffman DL, Salter JD, Brookes PS (2007) Am J Physiol Heart Circ Physiol
Abstract: Mitochondria are proposed to play an important role in hypoxic cell signaling. One currently accepted signaling paradigm is that the mitochondrial generation of reactive oxygen species (ROS) increases in hypoxia. This is paradoxical, because oxygen is a substrate for ROS generation. Although the response of isolated mitochondrial ROS generation to [O(2)] has been examined previously, such investigations did not apply rigorous control over [O(2)] within the hypoxic signaling range. With the use of open-flow respirometry and fluorimetry, the current study determined the response of isolated rat liver mitochondrial ROS generation to defined steady-state [O(2)] as low as 0.1 microM. In mitochondria respiring under state 4 (quiescent) or state 3 (ATP turnover) conditions, decreased ROS generation was always observed at low [O(2)]. It is concluded that the biochemical mechanism to facilitate increased ROS generation in response to hypoxia in cells is not intrinsic to the mitochondrial respiratory chain alone but may involve other factors. The implications for hypoxic cell signaling are discussed.
โข O2k-Network Lab: US_MI_Ann_Arbor_Glick_GD
Labels: MiParea: Respiration
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria Enzyme: Complex II;succinate dehydrogenase, Complex III Regulation: O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Redox state
- For a critical review on artefacts in oxygen kinetics in the paper by Hoffman et al (2007), see Scandurra_2010_Adv Exp Med Biol.