Gnaiger 2012 Abstract Bioblast-Gentle Science: Difference between revisions
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|event=[[Bioblast Conference on Mitochondrial Competence]] | |event=[[Bioblast Conference on Mitochondrial Competence]] | ||
|abstract=[[File:Gnaiger Erich.jpg|right|100px|Erich Gnaiger]] | |abstract=[[File:Gnaiger Erich.jpg|right|100px|Erich Gnaiger]] | ||
The concept of [[Gentle Science]] has been a driving force for more than 20 years of continuous development of [[high-resolution respirometry]], which was initiated in a [http://www.oroboros.at/?Oroboros1989 FWF project (1989)]. Our first generation [http://www.oroboros.at/?Oroboros1992 ''Oxygraph''] was commercially available in 1992. A series of applications was presented at a [[Gnaiger 1994 BTK|BTK conference in 1994]], and a full description of the instrument, method and concept of high-resolution respirometry was published in 1995 [1], when the positive feedback from early users had confirmed the validity of our approach [2]. The concept of MultiSensor extensions was implemented in the second generation Oxygraph-2k, developed in close cooperation with Phlipp Gradl (WGT-Elektronik) and distributed since [http://www.oroboros.at/?Oroboros2002 2002]. | The concept of [[Gentle Science]] has been a driving force for more than 20 years of continuous development of [[high-resolution respirometry]], which was initiated in a [http://www.oroboros.at/?Oroboros1989 FWF project (1989)]. Our first generation [http://www.oroboros.at/?Oroboros1992 ''Oxygraph''] was commercially available in 1992. A series of applications was presented at a [[Gnaiger 1994 BTK|BTK conference in 1994]], and a full description of the instrument, method and concept of high-resolution respirometry was published in 1995 [1], when the positive feedback from early users had confirmed the validity of our approach [2]. The concept of O2k-MultiSensor extensions was implemented in the second generation Oxygraph-2k, developed in close cooperation with Phlipp Gradl (WGT-Elektronik) and distributed since [http://www.oroboros.at/?Oroboros2002 2002]. | ||
The strategy of OROBOROS INSTRUMENTS included the close link between university and company, teaching and training with Open Access to our published [3] and unpublished scientific background information, detailed descriptions of [http://www.oroboros.at/?O2k-protocols O2k-Protocols] on our website, and direct communication with our customers and O2k-users ([[MiPNet_Reference_Laboratories|Mitochondrial Physiology Network]]). Β | The strategy of OROBOROS INSTRUMENTS included the close link between university and company, teaching and training with Open Access to our published [3] and unpublished scientific background information, detailed descriptions of [http://www.oroboros.at/?O2k-protocols O2k-Protocols] on our website, and direct communication with our customers and O2k-users ([[MiPNet_Reference_Laboratories|Mitochondrial Physiology Network]]). Β | ||
Conforming to the [http://www.oroboros.at/?Oroboros-logo OROBOROS logo] we are proud to receive highly positive [[O2k-Feedback]], but also provide help with [[O2k-Troubleshooting]] in open discussions. Since a [http://www.oroboros.at/?Mitopathways-group 2007 workshop in Schroecken] and the first [http://www.mitophysiology.org/?MiP-summerschool2007 MiPsummer school 2007], advanced SUIT protocols for sequential activation particularly of CI-, CI+II- and CII-linked respiration are explained in the '[[Gnaiger 2007 MitoPathways|Blue Book]]'. It is not in the spirit of Gentle Science, however, to receive enthusiastic feedback by Emails and then read in a publication (2008) ''protocols developed by us'' without proper citation. | |||
# [[Gnaiger 1995 J Bioenerg Biomembr|Gnaiger E, Steinlechner-Maran R, MΓ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]] | # [[Gnaiger 1995 J Bioenerg Biomembr|Gnaiger E, Steinlechner-Maran R, MΓ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]] | ||
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== Further references == | == Further references == | ||
* [[Gnaiger 1994 BTK|Gnaiger E, Gellerich FN, Wyss M (1994) What is Controlling Life? 50 years after Erwin SchrΓΆdinger's What is Life? Modern Trends in BiothermoKinetics 3, Innsbruck Univ Press, 336 pp.]] | * [[Gnaiger 1994 BTK|Gnaiger E, Gellerich FN, Wyss M (1994) What is Controlling Life? 50 years after Erwin SchrΓΆdinger's What is Life? Modern Trends in BiothermoKinetics 3, Innsbruck Univ Press, 336 pp.]] | ||
Β | * [[Gnaiger 2007 MitoPathways|Gnaiger E, ed (2007) Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck, 1st ed: 96 pp.]] | ||
== Help == | == Help == | ||
* [[Abstracts help]] | * [[Abstracts help]] | ||
* [[MitoPedia Glossary: Terms and abbreviations]] | * [[MitoPedia Glossary: Terms and abbreviations]] |
Revision as of 06:24, 23 October 2012
Gnaiger E (2012) Gentle Science and the Bioblast Conference. Mitochondr Physiol Network 17.12. |
Link: MiPNet17.12 Bioblast Conference - Open Access
Gnaiger E (2012)
Event: Bioblast Conference on Mitochondrial Competence
The concept of Gentle Science has been a driving force for more than 20 years of continuous development of high-resolution respirometry, which was initiated in a FWF project (1989). Our first generation Oxygraph was commercially available in 1992. A series of applications was presented at a BTK conference in 1994, and a full description of the instrument, method and concept of high-resolution respirometry was published in 1995 [1], when the positive feedback from early users had confirmed the validity of our approach [2]. The concept of O2k-MultiSensor extensions was implemented in the second generation Oxygraph-2k, developed in close cooperation with Phlipp Gradl (WGT-Elektronik) and distributed since 2002.
The strategy of OROBOROS INSTRUMENTS included the close link between university and company, teaching and training with Open Access to our published [3] and unpublished scientific background information, detailed descriptions of O2k-Protocols on our website, and direct communication with our customers and O2k-users (Mitochondrial Physiology Network).
Conforming to the OROBOROS logo we are proud to receive highly positive O2k-Feedback, but also provide help with O2k-Troubleshooting in open discussions. Since a 2007 workshop in Schroecken and the first MiPsummer school 2007, advanced SUIT protocols for sequential activation particularly of CI-, CI+II- and CII-linked respiration are explained in the 'Blue Book'. It is not in the spirit of Gentle Science, however, to receive enthusiastic feedback by Emails and then read in a publication (2008) protocols developed by us without proper citation.
- Gnaiger E, Steinlechner-Maran R, MΓ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.
- Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
β’ Keywords: High-resolution respirometry, Gentle Science, Oxygraph-2k
β’ O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck Gnaiger E
Labels:
Affiliations and author contributions
OROBOROS INSTRUMENTS, Innsbruck, Austria; and D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Austria. Email: [email protected]
Further references
- Gnaiger E, Gellerich FN, Wyss M (1994) What is Controlling Life? 50 years after Erwin SchrΓΆdinger's What is Life? Modern Trends in BiothermoKinetics 3, Innsbruck Univ Press, 336 pp.
- Gnaiger E, ed (2007) Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck, 1st ed: 96 pp.