- high-resolution terminology - matching measurements at high-resolution
Flux control efficiency
Description
Flux control capacities express the control of respiration by a metabolic variable, X, as a fractional change of flux from Y to Z, Ξj (normalized for Z, dimensionless). Z is the reference state with high (stimulated or un-inhibited) flux; Y is the background state at low flux, upon which X acts. This yields the flux control ratio jY=Y/Z),
- ΞjZ-Y = (Z-Y)/Z = 1-jY
Complementary to the concept of flux control ratios and analogous to elasticities of metabolic control analysis, the flux control capacity of X upon background Y is expressed as the change of flux from Y to Z normalized for the reference state Z.
Abbreviation: FCC
Reference: Gnaiger 2013 Abstract MiP2013
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property., "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Gnaiger E (2013)
- Flux control capacity. Bioblast-MitoPedia 2013-08-04.
Metabolic control variable and respiratory state
A metabolic control variable, X, is either added (stimulation, activation) or removed (reversal of inhibition) to yield a high flux in thereference state, Z, from the background state, Y. X, Y and Z denote the metabolic control variable (X) or respiratory state (Y, Z) and the corresponding respiratory fluxes, X=Z-Y. Experimentally, inhibitors are added rather than removed (-X); then Y is the background state in the presence of the inhibitor.
- X: Metabolic control variable acting on the background state, Y, to yield the reference state, Z. X stimulates or un-inhibits Y from low flux to Z at high flux.
- Y: The background state is the non-activated or inhibited respiratory state at low flux in relation to the reference state, Z. A metabolic control variable, X, acts on Y (substrate, activator) or is removed from Y (inhibitor) to yield Z.
- Z: The reference state, stimulated or un-inhibited by a metabolic control variable, X, with high flux in relation to the background state, Y.
Substrate control capacity
Substrate control capacities express the relative change of oxygen flux in response to a transition of substrate availability in a defined coupling state.
- CI and CII are abbreviations for Complex I and Complex II, but indicate here CI-linked respiration (with pyruvate, glutamate, malate, or other ETS competent CI-linked substrate combinations) and CII-linked (with succinate) respiration. CI+II indicates respiration with a CI- plus CII-linked substrate cocktail.
Coupling control capacity
Coupling control capacities are determined in an ETS-competent substrate state.
