From Bioblast
Fluorescence-Control Unit
| Description | Fluorescence-Control Unit with O2k-Front Fixation, Current-Control (O2k-Chamber A and B) for regulation of light intensity of the photodiodes in the fluorescence sensors. A component of the O2k-Fluorescence LED2-Module. |
|---|---|
| Product ID | 44100-01 |
| Type | O2k, MultiSensor, O2k-Fluorescence LED2-Module |
| Link | O2k-Fluorescence LED2-Module |
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Instructions
Setup of the O2k-Fluorescence LED2-Module
- Switch off the O2k with the power switch on the rear of the O2k-Main Unit.
- Remove both blue O2k-Window Frames. Insert the O2k-Window Tool around the outer rim of the window frame and unscrew counter clockwise.
- Remove the Sensor-Guide (‘nose’) from the O2k-Front Fixation of the Fluorescence-Control Unit. Loosen the small fixation screw and pull out the Sensor-Guide.
- Place the Fluorescence-Control Unit below the O2k-Chamber Block. Align the windows of the O2k-Front-Fixation with the windows of the O2k-Chambers, re-insert the blue O2k-Window Frames, and screw them finger-tight into the O2k-Main Unit.
- Re-attach the Sensor-Guide to the O2k-Front-Fixation and fasten the fixation screw finger-tight.
- Place the power-cables on the rear of the Fluorescence-Control Unit below the middle of the O2k-Main Unit from front to rear. Unplug the mains power cable of the O2k and plug it into the female plug of the Fluorescence-Control Unit. Insert the male plug of the Fluorescence-Control Unit into the mains socket at the rear of the O2k.
- Connect the amperometric cables attached to the side of the Fluorescence-Control Unit to the ‘Amp’ plugs (labelled "NO" in Series D-E) on the O2k-Main Unit.
In this configuration the O2k can be used for high-resolution respirometry and fluorometry. It is not necessary to dismount the Fluorescence-Control Unit for basic HRR when a fluorescence singal is not recorded.
Current-Control
The light intensity of the LEDs is controlled by changing the current supply independently for each fluorescence sensor (O2k-Chamber A and B).
- Position 0-8: off, 0.02, 0.5, 1, 2, 5, 10, 20, 30 mA. The polarization voltage of the Amp-Channel (NO-Channel) is set to zero in DatLab [Oxygraph]/[O2k-Control].
- Position 9: variable, controlled by the polarization voltage setting in DatLab [Oxygraph]/[O2k-Control].
