Difference between revisions of "FN"
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::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] | ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] | ||
::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]] | ::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]] | ||
::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 pce D007]] | ::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 ce-pce D007]] | ||
:::*[[SUIT-005]] | :::*[[SUIT-005]] | ||
::::* DL-Protocol for permeabilized fibers (pfi):[[SUIT-005 O2 pfi D011]] | ::::* DL-Protocol for permeabilized fibers (pfi):[[SUIT-005 O2 pfi D011]] | ||
Revision as of 16:18, 27 March 2019
- high-resolution terminology - matching measurements at high-resolution
FN
Description
FN is induced in mt-preparations by addition of NADH-generating substrates (N-pathway control state, or CI-linked pathway control) in combination with one or several fatty acids, which are supplied to feed electrons into the F-junction through fatty acyl CoA dehydrogenase (reduced form FADH2), to electron transferring flavoprotein (CETF), and further through the Q-junction to Complex III (CIII). FAO not only depends on electron transfer through the F-junction (which is typically rate-limiting), but simultaneously generates FADH2 and NADH and thus depends on N-junction throughput. Hence FAO can be inhibited completely by inhibition of Complex I (CI). This physiological substrate combination is required for partial reconstitution of TCA cycle function and convergent electron-input into the Q-junction, to compensate for metabolite depletion into the incubation medium. FS in combination exerts an additive effect of convergent electron flow in most types of mitochondria.
Abbreviation: FN
Reference: Electron transfer-pathway state, Gnaiger 2014 MitoPathways
MitoPedia concepts:
Respiratory state,
SUIT state,
Recommended
MitoPedia topics:
EAGLE
Communicated by Gnaiger E, edited 2019-01-22 by Komlodi T.
FNL
FN pathway in the LEAK state can be evaluated in the following SUIT protocols:
FNP
FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
- DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
- DL-Protocol for permeabilized cells (pce): SUIT-002 O2 ce-pce D007
- DL-Protocol for permeabilized fibers (pfi):SUIT-005 O2 pfi D011
-
FNE
FN pathway in the ET state can be evaluated in the following SUIT protocols:
