- high-resolution terminology - matching measurements at high-resolution
Electron-transfer-pathway state
Description
Substrate states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, tissue homogenate) by depletion of endogenous substrates and addition of defined substrates to the mitochondrial respiration medium. Mitochondrial substrate states have to be defined complementary to mitochondrial coupling states.
Specific substrate-inhibitor combinations are selected to establish substrate states for (i) stimulating defined segments of the electron transfer system, or (ii) reconstitution of TCA cycle function.
(i) The most commonly applied substrate states select for Complex I electron input (CI: pyruvate+malate, PM; glutamate+malate, GM), Complex II electron input (CII: succinate+rotenone, S(Rot)), or Complex IV electron input (CIV: ascorbate+TMPD(Ama)).
(ii) Reconstitution of TCA cycle function requires a CI+II-related substrate combination, such as PMS, GMS, or PMGS, applied simultaneously without inhibitor of any respiratory complexes.
Reference: Gnaiger_2009_IJBCB; List of respiratory states
Labels:
Enzyme: Complex I, Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property., Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Respiratory state"Respiratory state" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
