Difference between revisions of "D-number"

Revision as of 13:57, 5 August 2019

high-resolution terminology - matching measurements at high-resolution

D-number

Description

D number is the unique code given for each SUIT protocol. In the same SUIT protocol family (SUIT-###), there might be different protocols, specifically designed for different sample type (e.g., different mitochondrial preparations) or for different applications (e.g., O2, AmR, Fluo, MgG). Since the use of different kinds of sample or application may result in slightly different steps, each protocol receives a different D-number.

D-number in the SUIT-code

The D-number is shown at the end of the SUIT-code, e.g., SUIT-001_O2_mt_D001. For a list of SUIT protocols sorted by D-number, see the list of SUIT protocols with D-numbers

Example of different D-numbers with the same SUIT-number

The SUIT-001 family comprises different related protocols, each specifically designed for different sample preparations:

SUIT-001 O2 mt D001 for the following mitochondrial preparations: isolated mitochondria, tissue homogenates and cells that were permeabilized previously to the addition to the chamber;
SUIT-001 O2 pfi D002 for permeabilized fibers;
SUIT-001 O2 ce-pce D003 for living cells (ce) that will be further permeabilized inside the chamber (pce), with addition of digitonin;
SUIT-001 O2 ce-pce D004 for PBMCs and platelets, added in the chamber as living cells and further permeabilized inside the chamber.

Abbreviation: D###

MitoPedia concepts: SUIT concept

Labels: MiParea: Instruments;methods

HRR: Theory

@@ Line 15: / Line 15: @@
 For a list of SUIT protocols sorted by D-number, see the [[SUIT_protocol_library#List_of_SUIT_protocols_with_D-numbers|list of SUIT protocols with D-numbers]]
-[[File:SUIT-nomenclature.jpg|500px|SUIT protocols]]
+ [[File:SUIT-nomenclature.jpg|500px|SUIT protocols]]
 == Example of different D-numbers with the same SUIT-number ==
+::: The [[SUIT-001]] family comprises different related protocols, each specifically designed for different sample preparations:
+::::* [[SUIT-001 O2 mt D001]] for the following [[mitochondrial preparations]]: isolated mitochondria, tissue homogenates and cells that were permeabilized previously to the addition to the chamber;
+::::* [[SUIT-001 O2 pfi D002]] for [[Permeabilized muscle fibers|permeabilized fibers]];
+::::* [[SUIT-001 O2 ce-pce D003]] for [[living cells]] (ce) that will be further permeabilized inside the chamber (pce), with addition of [[digitonin]];
+::::* [[SUIT-001 O2 ce-pce D004]] for [[PBMC]]s and [[platelet]]s, added in the chamber as [[living cells]] and further permeabilized inside the chamber.